Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/30/afe94ec4f5f60ef39b3e43b91db11c/SkinN_FFPE_L02_RNA_01_B23LG7FLT4_1.starfusion.starfusion.fusion_predictions.tsv
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d4/b87bddd4c5051689f167bd8b2ae721/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d4/b87bddd4c5051689f167bd8b2ae721/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/stage-6ef41cd9-2e18-4687-842c-3f6f49c5643f/48/32e6a611973bf3895a673373080bf1/starfusion_filter_config.yaml
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/blacklist_breakpoint_database_2026-04-27.tsv
==> STAGING COMPLETE (6 inputs)

=== DEBUGGING INFORMATION ===
Current working directory: /tmp/nxf.psYRNkyirP
Input files:
  - starfusion_tsv: SkinN_FFPE_L02_RNA_01_B23LG7FLT4_1.starfusion.starfusion.fusion_predictions.tsv
  - blacklist: blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  - filter_config: starfusion_filter_config.yaml
  - aih_blacklist_breakpoints: blacklist_breakpoint_database_2026-04-27.tsv

=== PYTHON INFORMATION ===
/usr/local/bin/python
Python 3.11.14
/usr/local/bin/python3
Python 3.11.14

=== SCRIPT LOCATION ===
Checking for filter_starfusion_main.py:
/tmp/nxf.psYRNkyirP/nextflow-bin/filter_starfusion_main.py
filter_starfusion_main.py not in current directory
bin/filter_starfusion_main.py not found
bin/ directory does not exist

=== ENVIRONMENT PATH ===
/tmp/nxf.psYRNkyirP/nextflow-bin:/usr/local/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin

=== CURRENT DIRECTORY CONTENTS ===
total 64088
drwx------. 3 1000 root     4096 May 28 22:12 .
drwxrwxrwx. 4 root root       86 May 28 22:12 ..
-rw-r--r--. 1 1000 root        0 May 28 22:12 .command.err
-rw-r--r--. 1 1000 root      885 May 28 22:12 .command.out
-rw-r--r--. 1 1000 root    14593 May 28 22:12 .command.run
-rw-r--r--. 1 1000 root     3242 May 28 22:12 .command.sh
-rw-r--r--. 1 1000 root        0 May 28 22:12 .command.trace
-rw-r--r--. 1 1000 root    89253 May 28 22:12 SkinN_FFPE_L02_RNA_01_B23LG7FLT4_1.starfusion.starfusion.fusion_predictions.tsv
-rw-r--r--. 1 1000 root 10714998 May 28 22:12 blacklist_breakpoint_database_2026-04-27.tsv
-rw-r--r--. 1 1000 root 54779787 May 28 22:12 blacklist_hg38_GRCh38_v2.4.0.tsv.gz
drwxr-xr-x. 4 1000 root     4096 May 28 22:12 nextflow-bin
-rw-r--r--. 1 1000 root     4132 May 28 22:12 starfusion_filter_config.yaml

=== PROJECT DIRECTORY INFO ===
NXF_PROJECT_DIR: not set
baseDir: not set

=== Running filter script ===

================================================================================
STEP 1: LOADING STAR-FUSION PREDICTIONS
================================================================================
Loading STAR-Fusion predictions from: SkinN_FFPE_L02_RNA_01_B23LG7FLT4_1.starfusion.starfusion.fusion_predictions.tsv
  ✓ Loaded: 297 fusions

================================================================================
STEP 2: CONVERTING TO BREAKPOINT_ID FORMAT
================================================================================
✓ Converted to breakpoint_ID format: 297 valid, 0 invalid

================================================================================
STEP 3: FLAGGING WITH BLACKLIST
================================================================================
Loading blacklist (this may take a while)...
Loading blacklist from: blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  Loaded 9381 single rules and 16721785 pair rules
  Indexed blacklist (bin_size=100000)
Flagging fusions with blacklist...
✓ Flagged 16 of 297 fusions as blacklisted

================================================================================
STEP 3b: AIH CURATED BREAKPOINT BLACKLIST
================================================================================
Loading AIH blacklist table from: blacklist_breakpoint_database_2026-04-27.tsv
  Qualified breakpoint keys (orientations): 1378
  AIH curated blacklist: matched 19 fusion rows (breakpoint_ID in qualified set)

================================================================================
STEP 4: APPLYING YAML-BASED FILTER
================================================================================
✓ Filter initialized from: starfusion_filter_config.yaml
Applying filter criteria (adding CALL column)...
✓ Filter applied: 0 pass (CALL=1), 297 fail (CALL=0)
  Total fusions: 297 (all rows retained)
  Added FILTER column: PASS=0, FAIL=297

✓ Verified: All 27 blacklisted fusions have CALL=0

================================================================================
STEP 5: GENERATING SUMMARY STATISTICS
================================================================================

================================================================================
FILTERING SUMMARY
================================================================================

Total fusions: 297
  Pass (CALL=1): 0 (0.0%)
  Fail (CALL=0): 297 (100.0%)

Blacklist Flagging:
  Total blacklisted: 27
  ✓ All 27 blacklisted fusions have CALL=0

Failure Reasons (top 10):
  Excluded SpliceType: INCL_NON_REF_SPLICE: 204
  Read count too low: 1 < 8: 49
  aih_curated_blacklist score(3): 11
  Excluded annotation pattern: HGNC_GENEFAM: 11
  Blacklisted; aih_curated_blacklist score(3): 8
  Blacklisted: 8
  Read count too low: 2 < 8: 4
  Excluded annotation pattern: DGD_PARALOGS: 1
  Excluded annotation pattern: GTEx_recurrent_StarF2019: 1

Breakdown by SpliceType:
  INCL_NON_REF_SPLICE: 0 pass, 244 fail (total: 244)
  ONLY_REF_SPLICE: 0 pass, 53 fail (total: 53)

Read Count Statistics:
  Fusions with < 3 total reads: 289
  Of these, 289 have CALL=0

================================================================================
STEP 6: EXPORTING RESULTS
================================================================================
✓ Results exported to: SkinN_FFPE_L02_RNA_01_B23LG7FLT4_1.starfusion.starfusion.fusion_predictions.filtered.tsv
  Total fusions: 297
  CALL=1 (pass): 0
  CALL=0 (fail): 297
✓ Empty pass_only file created (no passing fusions): SkinN_FFPE_L02_RNA_01_B23LG7FLT4_1.starfusion.starfusion.fusion_predictions.filtered_pass_only.tsv
✓ Summary exported to: SkinN_FFPE_L02_RNA_01_B23LG7FLT4_1.starfusion.starfusion.fusion_predictions.filter_summary.txt

================================================================================
✓ FILTERING COMPLETE
================================================================================