+ mkdir A673_FFPE_RNA_0001_B23LG7FLT4_1 + all_qc -bam A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder A673_FFPE_RNA_0001_B23LG7FLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai 0it [00:00, ?it/s] 83941it [00:00, 839318.75it/s] 171038it [00:00, 857900.69it/s] 258576it [00:00, 865851.01it/s] 345162it [00:00, 857366.51it/s] 436816it [00:00, 878508.76it/s] 524689it [00:00, 867182.54it/s] 611445it [00:00, 801665.39it/s] 695044it [00:00, 811911.41it/s] 784116it [00:00, 835479.84it/s] 874861it [00:01, 857008.87it/s] 964995it [00:01, 870275.67it/s] 1053559it [00:01, 874861.56it/s] 1142948it [00:01, 880526.18it/s] 1232099it [00:01, 883797.41it/s] 1320603it [00:01, 862538.83it/s] 1407049it [00:01, 832783.23it/s] 1490634it [00:01, 832061.83it/s] 1574052it [00:01, 823174.48it/s] 1659077it [00:01, 831056.24it/s] 1747146it [00:02, 845606.88it/s] 1831824it [00:02, 809438.42it/s] 1913140it [00:02, 796646.72it/s] 2004992it [00:02, 831721.15it/s] 2096466it [00:02, 855896.59it/s] 2185046it [00:02, 864666.03it/s] 2274669it [00:02, 873978.24it/s] 2363485it [00:02, 878171.83it/s] 2455376it [00:02, 890289.23it/s] 2544502it [00:02, 887400.54it/s] 2633311it [00:03, 879097.26it/s] 2725829it [00:03, 892750.85it/s] 2815163it [00:03, 886744.67it/s] 2906045it [00:03, 893278.96it/s] 2995412it [00:03, 890815.39it/s] 3084521it [00:03, 888949.15it/s] 3173435it [00:03, 862097.42it/s] 3264759it [00:03, 876981.17it/s] 3354457it [00:03, 882855.77it/s] 3442867it [00:04, 876098.72it/s] 3531396it [00:04, 878794.16it/s] 3619629it [00:04, 879823.01it/s] 3707660it [00:04, 841658.61it/s] 3795703it [00:04, 852856.89it/s] 3881286it [00:04, 837900.65it/s] 3965311it [00:04, 791109.24it/s] 4045023it [00:04, 778267.36it/s] 4123248it [00:04, 776180.25it/s] 4206272it [00:04, 791681.95it/s] 4288342it [00:05, 800104.55it/s] 4369804it [00:05, 804345.70it/s] 4452342it [00:05, 810542.43it/s] 4541262it [00:05, 833872.74it/s] 4629242it [00:05, 847529.19it/s] 4719585it [00:05, 864192.25it/s] 4809733it [00:05, 875308.98it/s] 4897317it [00:05, 861007.04it/s] 4995536it [00:05, 896857.77it/s] 5089404it [00:05, 909273.65it/s] 5180420it [00:06, 894836.35it/s] 5270009it [00:06, 877392.25it/s] 5363822it [00:06, 895135.85it/s] 5453470it [00:06, 891128.38it/s] 5543134it [00:06, 892735.70it/s] 5632474it [00:06, 881993.74it/s] 5720739it [00:06, 854482.87it/s] 5809698it [00:06, 864645.77it/s] 5897532it [00:06, 868639.62it/s] 5984526it [00:06, 854407.92it/s] 6076423it [00:07, 873299.85it/s] 6163885it [00:07, 864580.56it/s] 6250442it [00:07, 855927.31it/s] 6336109it [00:07, 853889.39it/s] 6423841it [00:07, 860784.79it/s] 6509965it [00:07, 856838.63it/s] 6595681it [00:07, 851692.23it/s] 6687070it [00:07, 870107.04it/s] 6778581it [00:07, 883475.41it/s] 6869811it [00:08, 892055.36it/s] 6960357it [00:08, 896041.98it/s] 7053112it [00:08, 905446.23it/s] 7143677it [00:08, 897274.88it/s] 7233434it [00:08, 889628.17it/s] 7322427it [00:08, 851487.90it/s] 7407910it [00:08, 801001.94it/s] 7488723it [00:08, 766320.45it/s] 7565988it [00:08, 758762.28it/s] 7652616it [00:08, 788856.99it/s] 7740864it [00:09, 815592.60it/s] 7829222it [00:09, 835202.55it/s] 7920462it [00:09, 857764.76it/s] 8008949it [00:09, 865739.63it/s] 8099481it [00:09, 877452.73it/s] 8187416it [00:09, 850694.64it/s] 8272780it [00:09, 847353.90it/s] 8359338it [00:09, 852679.91it/s] 8444759it [00:09, 851704.22it/s] 8535894it [00:09, 869345.80it/s] 8622926it [00:10, 862206.86it/s] 8709223it [00:10, 852551.05it/s] 8794545it [00:10, 846566.46it/s] 8879249it [00:10, 819492.89it/s] 8966911it [00:10, 836021.70it/s] 9050701it [00:10, 835475.58it/s] 9137095it [00:10, 843840.97it/s] 9221586it [00:10, 829260.33it/s] 9309522it [00:10, 843954.58it/s] 9398708it [00:11, 858096.84it/s] 9484618it [00:11, 857557.34it/s] 9570444it [00:11, 842449.75it/s] 9654787it [00:11, 788601.60it/s] 9734348it [00:11, 762004.80it/s] 9817376it [00:11, 781107.41it/s] 9896017it [00:11, 782503.94it/s] 9988338it [00:11, 823267.08it/s] 10072869it [00:11, 829703.87it/s] 10156141it [00:11, 809721.66it/s] 10237405it [00:12, 772428.03it/s] 10315134it [00:12, 747120.25it/s] 10390257it [00:12, 733060.12it/s] 10471923it [00:12, 756665.31it/s] 10553976it [00:12, 775002.31it/s] 10640537it [00:12, 801367.48it/s] 10724645it [00:12, 813012.96it/s] 10810478it [00:12, 826394.16it/s] 10900126it [00:12, 847106.33it/s] 10989659it [00:12, 861400.28it/s] 11075915it [00:13, 846156.01it/s] 11166401it [00:13, 863458.81it/s] 11253564it [00:13, 865864.42it/s] 11340240it [00:13, 865556.39it/s] 11430244it [00:13, 875815.05it/s] 11520059it [00:13, 882470.42it/s] 11608344it [00:13, 867022.79it/s] 11695130it [00:13, 833945.36it/s] 11778816it [00:13, 827750.28it/s] 11865934it [00:14, 840344.15it/s] 11950146it [00:14, 837498.47it/s] 12034016it [00:14, 836697.39it/s] 12127017it [00:14, 864138.12it/s] 12217318it [00:14, 875660.24it/s] 12306184it [00:14, 879511.41it/s] 12394194it [00:14, 878464.51it/s] 12482081it [00:14, 877368.26it/s] 12570984it [00:14, 880831.94it/s] 12661160it [00:14, 887070.27it/s] 12749884it [00:15, 881621.94it/s] 12838065it [00:15, 872874.59it/s] 12925381it [00:15, 850668.03it/s] 13014946it [00:15, 863691.60it/s] 13102200it [00:15, 866279.75it/s] 13188917it [00:15, 857374.13it/s] 13276364it [00:15, 862396.50it/s] 13362663it [00:15, 846009.13it/s] 13451815it [00:15, 859353.57it/s] 13537846it [00:15, 855840.35it/s] 13625162it [00:16, 860946.69it/s] 13712618it [00:16, 864976.62it/s] 13799157it [00:16, 860622.99it/s] 13892289it [00:16, 881631.02it/s] 13980492it [00:16, 855919.64it/s] 14071064it [00:16, 870445.38it/s] 14158427it [00:16, 871368.35it/s] 14247493it [00:16, 877067.34it/s] 14337643it [00:16, 884314.32it/s] 14426143it [00:16, 868598.65it/s] 14515243it [00:17, 875188.36it/s] 14550647it [00:17, 850315.20it/s] [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.md.bai + merge_qc -folders A673_FFPE_RNA_0001_B23LG7FLT4_1/A673_FFPE_RNA_0001_B23LG7FLT4_1.md -output A673_FFPE_RNA_0001_B23LG7FLT4_1/A673_FFPE_RNA_0001_B23LG7FLT4_1_full_basic_stats.tsv + head -n1 A673_FFPE_RNA_0001_B23LG7FLT4_1/A673_FFPE_RNA_0001_B23LG7FLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed A673_FFPE_RNA_0001_B23LG7FLT4_1/A673_FFPE_RNA_0001_B23LG7FLT4_1_full_basic_stats.tsv