[W::hts_idx_load3] The index file is older than the data file: A549_FFPE_01_RNA_0001_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (28) splicing reads. 14 splicing junctions. 2 known splicing junctions. 12 novel splicing junctions.
sampling 10% (56) splicing reads. 21 splicing junctions. 2 known splicing junctions. 19 novel splicing junctions.
sampling 15% (85) splicing reads. 29 splicing junctions. 3 known splicing junctions. 26 novel splicing junctions.
sampling 20% (113) splicing reads. 42 splicing junctions. 5 known splicing junctions. 37 novel splicing junctions.
sampling 25% (141) splicing reads. 45 splicing junctions. 5 known splicing junctions. 40 novel splicing junctions.
sampling 30% (170) splicing reads. 54 splicing junctions. 6 known splicing junctions. 48 novel splicing junctions.
sampling 35% (198) splicing reads. 58 splicing junctions. 7 known splicing junctions. 51 novel splicing junctions.
sampling 40% (226) splicing reads. 65 splicing junctions. 7 known splicing junctions. 58 novel splicing junctions.
sampling 45% (255) splicing reads. 69 splicing junctions. 9 known splicing junctions. 60 novel splicing junctions.
sampling 50% (283) splicing reads. 72 splicing junctions. 10 known splicing junctions. 62 novel splicing junctions.
sampling 55% (311) splicing reads. 75 splicing junctions. 10 known splicing junctions. 65 novel splicing junctions.
sampling 60% (340) splicing reads. 80 splicing junctions. 12 known splicing junctions. 68 novel splicing junctions.
sampling 65% (368) splicing reads. 82 splicing junctions. 12 known splicing junctions. 70 novel splicing junctions.
sampling 70% (396) splicing reads. 86 splicing junctions. 12 known splicing junctions. 74 novel splicing junctions.
sampling 75% (425) splicing reads. 88 splicing junctions. 14 known splicing junctions. 74 novel splicing junctions.
sampling 80% (453) splicing reads. 88 splicing junctions. 14 known splicing junctions. 74 novel splicing junctions.
sampling 85% (481) splicing reads. 92 splicing junctions. 15 known splicing junctions. 77 novel splicing junctions.
sampling 90% (510) splicing reads. 93 splicing junctions. 15 known splicing junctions. 78 novel splicing junctions.
sampling 95% (538) splicing reads. 93 splicing junctions. 15 known splicing junctions. 78 novel splicing junctions.
sampling 100% (567) splicing reads. 94 splicing junctions. 15 known splicing junctions. 79 novel splicing junctions.