[W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (1427205) splicing reads. 100142 splicing junctions. 95055 known splicing junctions. 5087 novel splicing junctions.
sampling 10% (2854410) splicing reads. 108779 splicing junctions. 102286 known splicing junctions. 6493 novel splicing junctions.
sampling 15% (4281616) splicing reads. 112361 splicing junctions. 104942 known splicing junctions. 7419 novel splicing junctions.
sampling 20% (5708821) splicing reads. 114401 splicing junctions. 106332 known splicing junctions. 8069 novel splicing junctions.
sampling 25% (7136027) splicing reads. 115868 splicing junctions. 107231 known splicing junctions. 8637 novel splicing junctions.
sampling 30% (8563232) splicing reads. 116957 splicing junctions. 107862 known splicing junctions. 9095 novel splicing junctions.
sampling 35% (9990437) splicing reads. 117809 splicing junctions. 108311 known splicing junctions. 9498 novel splicing junctions.
sampling 40% (11417643) splicing reads. 118520 splicing junctions. 108688 known splicing junctions. 9832 novel splicing junctions.
sampling 45% (12844848) splicing reads. 119097 splicing junctions. 108988 known splicing junctions. 10109 novel splicing junctions.
sampling 50% (14272054) splicing reads. 119588 splicing junctions. 109228 known splicing junctions. 10360 novel splicing junctions.
sampling 55% (15699259) splicing reads. 120024 splicing junctions. 109438 known splicing junctions. 10586 novel splicing junctions.
sampling 60% (17126464) splicing reads. 120342 splicing junctions. 109574 known splicing junctions. 10768 novel splicing junctions.
sampling 65% (18553670) splicing reads. 120666 splicing junctions. 109711 known splicing junctions. 10955 novel splicing junctions.
sampling 70% (19980875) splicing reads. 120946 splicing junctions. 109839 known splicing junctions. 11107 novel splicing junctions.
sampling 75% (21408081) splicing reads. 121175 splicing junctions. 109945 known splicing junctions. 11230 novel splicing junctions.
sampling 80% (22835286) splicing reads. 121329 splicing junctions. 110010 known splicing junctions. 11319 novel splicing junctions.
sampling 85% (24262491) splicing reads. 121484 splicing junctions. 110072 known splicing junctions. 11412 novel splicing junctions.
sampling 90% (25689697) splicing reads. 121586 splicing junctions. 110107 known splicing junctions. 11479 novel splicing junctions.
sampling 95% (27116902) splicing reads. 121657 splicing junctions. 110135 known splicing junctions. 11522 novel splicing junctions.
sampling 100% (28544108) splicing reads. 121677 splicing junctions. 110143 known splicing junctions. 11534 novel splicing junctions.