[W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15317119) splicing reads. 246491 splicing junctions. 167836 known splicing junctions. 78655 novel splicing junctions.
sampling 10% (30634239) splicing reads. 308065 splicing junctions. 180912 known splicing junctions. 127153 novel splicing junctions.
sampling 15% (45951359) splicing reads. 352672 splicing junctions. 188043 known splicing junctions. 164629 novel splicing junctions.
sampling 20% (61268479) splicing reads. 388515 splicing junctions. 192828 known splicing junctions. 195687 novel splicing junctions.
sampling 25% (76585599) splicing reads. 418308 splicing junctions. 196356 known splicing junctions. 221952 novel splicing junctions.
sampling 30% (91902719) splicing reads. 444374 splicing junctions. 199269 known splicing junctions. 245105 novel splicing junctions.
sampling 35% (107219839) splicing reads. 466686 splicing junctions. 201500 known splicing junctions. 265186 novel splicing junctions.
sampling 40% (122536959) splicing reads. 486292 splicing junctions. 203300 known splicing junctions. 282992 novel splicing junctions.
sampling 45% (137854079) splicing reads. 503702 splicing junctions. 204819 known splicing junctions. 298883 novel splicing junctions.
sampling 50% (153171199) splicing reads. 518863 splicing junctions. 206140 known splicing junctions. 312723 novel splicing junctions.
sampling 55% (168488319) splicing reads. 532493 splicing junctions. 207294 known splicing junctions. 325199 novel splicing junctions.
sampling 60% (183805439) splicing reads. 544514 splicing junctions. 208213 known splicing junctions. 336301 novel splicing junctions.
sampling 65% (199122559) splicing reads. 555112 splicing junctions. 209010 known splicing junctions. 346102 novel splicing junctions.
sampling 70% (214439679) splicing reads. 564662 splicing junctions. 209745 known splicing junctions. 354917 novel splicing junctions.
sampling 75% (229756799) splicing reads. 572877 splicing junctions. 210338 known splicing junctions. 362539 novel splicing junctions.
sampling 80% (245073919) splicing reads. 580196 splicing junctions. 210871 known splicing junctions. 369325 novel splicing junctions.
sampling 85% (260391039) splicing reads. 586374 splicing junctions. 211304 known splicing junctions. 375070 novel splicing junctions.
sampling 90% (275708159) splicing reads. 591558 splicing junctions. 211632 known splicing junctions. 379926 novel splicing junctions.
sampling 95% (291025279) splicing reads. 595906 splicing junctions. 211936 known splicing junctions. 383970 novel splicing junctions.
sampling 100% (306342399) splicing reads. 599313 splicing junctions. 212163 known splicing junctions. 387150 novel splicing junctions.