reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15) splicing reads. 8 splicing junctions. 3 known splicing junctions. 5 novel splicing junctions.
sampling 10% (30) splicing reads. 16 splicing junctions. 4 known splicing junctions. 12 novel splicing junctions.
sampling 15% (45) splicing reads. 19 splicing junctions. 4 known splicing junctions. 15 novel splicing junctions.
sampling 20% (60) splicing reads. 19 splicing junctions. 4 known splicing junctions. 15 novel splicing junctions.
sampling 25% (75) splicing reads. 21 splicing junctions. 4 known splicing junctions. 17 novel splicing junctions.
sampling 30% (90) splicing reads. 24 splicing junctions. 4 known splicing junctions. 20 novel splicing junctions.
sampling 35% (105) splicing reads. 28 splicing junctions. 4 known splicing junctions. 24 novel splicing junctions.
sampling 40% (120) splicing reads. 29 splicing junctions. 4 known splicing junctions. 25 novel splicing junctions.
sampling 45% (135) splicing reads. 29 splicing junctions. 4 known splicing junctions. 25 novel splicing junctions.
sampling 50% (151) splicing reads. 30 splicing junctions. 4 known splicing junctions. 26 novel splicing junctions.
sampling 55% (166) splicing reads. 30 splicing junctions. 4 known splicing junctions. 26 novel splicing junctions.
sampling 60% (181) splicing reads. 30 splicing junctions. 4 known splicing junctions. 26 novel splicing junctions.
sampling 65% (196) splicing reads. 32 splicing junctions. 4 known splicing junctions. 28 novel splicing junctions.
sampling 70% (211) splicing reads. 32 splicing junctions. 4 known splicing junctions. 28 novel splicing junctions.
sampling 75% (226) splicing reads. 32 splicing junctions. 4 known splicing junctions. 28 novel splicing junctions.
sampling 80% (241) splicing reads. 32 splicing junctions. 4 known splicing junctions. 28 novel splicing junctions.
sampling 85% (256) splicing reads. 33 splicing junctions. 4 known splicing junctions. 29 novel splicing junctions.
sampling 90% (271) splicing reads. 33 splicing junctions. 4 known splicing junctions. 29 novel splicing junctions.
sampling 95% (286) splicing reads. 33 splicing junctions. 4 known splicing junctions. 29 novel splicing junctions.
sampling 100% (302) splicing reads. 35 splicing junctions. 4 known splicing junctions. 31 novel splicing junctions.