[W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15771963) splicing reads. 251187 splicing junctions. 168834 known splicing junctions. 82353 novel splicing junctions.
sampling 10% (31543926) splicing reads. 314906 splicing junctions. 181756 known splicing junctions. 133150 novel splicing junctions.
sampling 15% (47315889) splicing reads. 361703 splicing junctions. 189007 known splicing junctions. 172696 novel splicing junctions.
sampling 20% (63087852) splicing reads. 399378 splicing junctions. 193977 known splicing junctions. 205401 novel splicing junctions.
sampling 25% (78859815) splicing reads. 431073 splicing junctions. 197559 known splicing junctions. 233514 novel splicing junctions.
sampling 30% (94631778) splicing reads. 458515 splicing junctions. 200370 known splicing junctions. 258145 novel splicing junctions.
sampling 35% (110403742) splicing reads. 482299 splicing junctions. 202639 known splicing junctions. 279660 novel splicing junctions.
sampling 40% (126175705) splicing reads. 503169 splicing junctions. 204534 known splicing junctions. 298635 novel splicing junctions.
sampling 45% (141947668) splicing reads. 521526 splicing junctions. 206091 known splicing junctions. 315435 novel splicing junctions.
sampling 50% (157719631) splicing reads. 538108 splicing junctions. 207461 known splicing junctions. 330647 novel splicing junctions.
sampling 55% (173491594) splicing reads. 552771 splicing junctions. 208583 known splicing junctions. 344188 novel splicing junctions.
sampling 60% (189263557) splicing reads. 565821 splicing junctions. 209602 known splicing junctions. 356219 novel splicing junctions.
sampling 65% (205035520) splicing reads. 577611 splicing junctions. 210445 known splicing junctions. 367166 novel splicing junctions.
sampling 70% (220807484) splicing reads. 587943 splicing junctions. 211116 known splicing junctions. 376827 novel splicing junctions.
sampling 75% (236579447) splicing reads. 597042 splicing junctions. 211714 known splicing junctions. 385328 novel splicing junctions.
sampling 80% (252351410) splicing reads. 604841 splicing junctions. 212239 known splicing junctions. 392602 novel splicing junctions.
sampling 85% (268123373) splicing reads. 611680 splicing junctions. 212702 known splicing junctions. 398978 novel splicing junctions.
sampling 90% (283895336) splicing reads. 617382 splicing junctions. 213084 known splicing junctions. 404298 novel splicing junctions.
sampling 95% (299667299) splicing reads. 622189 splicing junctions. 213397 known splicing junctions. 408792 novel splicing junctions.
sampling 100% (315439263) splicing reads. 626028 splicing junctions. 213632 known splicing junctions. 412396 novel splicing junctions.