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rate=28434782.61/min [21810000], rate=28447826.09/min [21820000], rate=28460869.57/min [21830000], rate=28473913.04/min [21840000], rate=28486956.52/min [21850000], rate=28500000.00/min [21860000], rate=28513043.48/min [21870000], rate=28526086.96/min [21880000], rate=28539130.43/min [21890000], rate=28552173.91/min [21900000], rate=28565217.39/min [21910000], rate=28578260.87/min [21920000], rate=28591304.35/min [21930000], rate=28604347.83/min [21940000], rate=28617391.30/min [21950000], rate=28630434.78/min [21960000], rate=28643478.26/min [21970000], rate=28656521.74/min [21980000], rate=28669565.22/min [21990000], rate=28682608.70/min [22000000], rate=28695652.17/min [22010000], rate=28708695.65/min [22020000], rate=28721739.13/min [22030000], rate=28734782.61/min [22040000], rate=28747826.09/min [22050000], rate=28760869.57/min [22060000], rate=28773913.04/min [22070000], rate=28786956.52/min [22080000], rate=28800000.00/min [22090000], rate=28813043.48/min [22100000], rate=28826086.96/min [22110000], rate=28839130.43/min [22120000], rate=28852173.91/min [22130000], rate=28865217.39/min [22140000], rate=28878260.87/min [22150000], rate=28891304.35/min [22160000], rate=28904347.83/min [22170000], rate=28917391.30/min [22180000], rate=28930434.78/min [22190000], rate=28943478.26/min [22200000], rate=28956521.74/min [22210000], rate=28353191.49/min [22220000], rate=28365957.45/min [22230000], rate=28378723.40/min [22240000], rate=28391489.36/min [22250000], rate=28404255.32/min [22260000], rate=28417021.28/min [22270000], rate=28429787.23/min [22280000], rate=28442553.19/min [22290000], rate=28455319.15/min [22300000], rate=28468085.11/min [22310000], rate=28480851.06/min [22320000], rate=28493617.02/min [22330000], rate=28506382.98/min [22340000], rate=28519148.94/min [22350000], rate=28531914.89/min [22360000], rate=28544680.85/min [22370000], rate=28557446.81/min [22380000], rate=28570212.77/min [22390000], rate=28582978.72/min [22400000], rate=28595744.68/min [22410000], rate=28608510.64/min [22420000], rate=28621276.60/min 08:45:09 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer.introns.prelim Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18. -OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.introns: identified 1205 cancer introns 08:45:09 : INFO : Execution Time = 0.00 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer.introns.prelim 08:45:09 : INFO : Running: touch /tmp/nxf.BYqmF8am6C/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.chckpts/prelim_introns.ok 08:45:09 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer.introns.prelim --min_total_reads 5 > OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer.introns 08:45:10 : INFO : Execution Time = 0.00 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer.introns.prelim --min_total_reads 5 > OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer.introns 08:45:10 : INFO : Running: touch /tmp/nxf.BYqmF8am6C/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.chckpts/introns_filtered.ok 08:45:10 : INFO : -found 893 cancer introns 08:45:10 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.introns --cancer_introns OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed 08:45:10 : INFO : Creating the BED File. 08:45:12 : INFO : Saving Bed File as OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed 08:45:12 : INFO : Execution Time = 0.04 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.introns --cancer_introns OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed 08:45:12 : INFO : Running: touch /tmp/nxf.BYqmF8am6C/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.chckpts/intron_igv_bed.ok 08:45:12 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed --bam OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam --output_prefix OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1 [W::hts_idx_load3] The index file is older than the data file: OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam.bai 08:49:57 : INFO : Execution Time = 4.75 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed --bam OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam --output_prefix OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1 08:49:57 : INFO : Running: touch /tmp/nxf.BYqmF8am6C/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.chckpts/reads_alignments_extracted.ok 08:49:57 : INFO : Running: samtools sort -o OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer_intron_reads.sorted.bam OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer_intron_reads.bam 08:49:58 : INFO : Execution Time = 0.00 minutes. CMD: samtools sort -o OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer_intron_reads.sorted.bam OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer_intron_reads.bam 08:49:58 : INFO : Running: touch /tmp/nxf.BYqmF8am6C/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.chckpts/sort_cancer_intron_reads.ok 08:49:58 : INFO : Running: samtools sort -o OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.bam OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.bam [bam_sort_core] merging from 33 files and 1 in-memory blocks... 08:55:46 : INFO : Execution Time = 5.81 minutes. CMD: samtools sort -o OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.bam OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.bam 08:55:46 : INFO : Running: touch /tmp/nxf.BYqmF8am6C/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.chckpts/sort_gene_reads.ok 08:55:46 : INFO : Running: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.tmp --keep_secondary OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.bam 08:58:10 : INFO : Execution Time = 2.40 minutes. CMD: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.tmp --keep_secondary OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.bam 08:58:10 : INFO : Running: touch /tmp/nxf.BYqmF8am6C/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.chckpts/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.tmp.ok 08:58:10 : INFO : Running: samtools sort -o OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.tmp [bam_sort_core] merging from 1 files and 1 in-memory blocks... 08:58:28 : INFO : Execution Time = 0.30 minutes. CMD: samtools sort -o OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.tmp 08:58:28 : INFO : Running: touch /tmp/nxf.BYqmF8am6C/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.chckpts/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.tmpsorted.ok 08:58:28 : INFO : Running: samtools index OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam 08:58:30 : INFO : Execution Time = 0.04 minutes. CMD: samtools index OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam 08:58:30 : INFO : Running: touch /tmp/nxf.BYqmF8am6C/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.chckpts/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.indexed.ok 08:58:30 : INFO : Running: samtools index OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer_intron_reads.sorted.bam 08:58:30 : INFO : Execution Time = 0.00 minutes. CMD: samtools index OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer_intron_reads.sorted.bam 08:58:30 : INFO : Running: touch /tmp/nxf.BYqmF8am6C/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.chckpts/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.cancer_intron_reads.sorted.bam.indexed.ok 08:58:30 : INFO : Running: create_report OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.ctat-splicing.igv.html --track-config OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1' 09:00:02 : INFO : Execution Time = 1.53 minutes. CMD: create_report OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.ctat-splicing.igv.html --track-config OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1' 09:00:02 : INFO : Running: touch /tmp/nxf.BYqmF8am6C/OvarianN_FFPE_L04_RNA_01_B23LG7FLT4_1.chckpts/igv_create_html.ok 09:00:02 : INFO : done.