[W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_3.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15729307) splicing reads. 249330 splicing junctions. 170096 known splicing junctions. 79234 novel splicing junctions.
sampling 10% (31458615) splicing reads. 310176 splicing junctions. 182704 known splicing junctions. 127472 novel splicing junctions.
sampling 15% (47187923) splicing reads. 355169 splicing junctions. 189800 known splicing junctions. 165369 novel splicing junctions.
sampling 20% (62917231) splicing reads. 391294 splicing junctions. 194626 known splicing junctions. 196668 novel splicing junctions.
sampling 25% (78646539) splicing reads. 421340 splicing junctions. 198127 known splicing junctions. 223213 novel splicing junctions.
sampling 30% (94375847) splicing reads. 447734 splicing junctions. 200973 known splicing junctions. 246761 novel splicing junctions.
sampling 35% (110105154) splicing reads. 470450 splicing junctions. 203269 known splicing junctions. 267181 novel splicing junctions.
sampling 40% (125834462) splicing reads. 490458 splicing junctions. 205113 known splicing junctions. 285345 novel splicing junctions.
sampling 45% (141563770) splicing reads. 508296 splicing junctions. 206692 known splicing junctions. 301604 novel splicing junctions.
sampling 50% (157293078) splicing reads. 524035 splicing junctions. 208016 known splicing junctions. 316019 novel splicing junctions.
sampling 55% (173022386) splicing reads. 538118 splicing junctions. 209124 known splicing junctions. 328994 novel splicing junctions.
sampling 60% (188751694) splicing reads. 550268 splicing junctions. 210025 known splicing junctions. 340243 novel splicing junctions.
sampling 65% (204481002) splicing reads. 561400 splicing junctions. 210832 known splicing junctions. 350568 novel splicing junctions.
sampling 70% (220210309) splicing reads. 571036 splicing junctions. 211569 known splicing junctions. 359467 novel splicing junctions.
sampling 75% (235939617) splicing reads. 579613 splicing junctions. 212172 known splicing junctions. 367441 novel splicing junctions.
sampling 80% (251668925) splicing reads. 587075 splicing junctions. 212684 known splicing junctions. 374391 novel splicing junctions.
sampling 85% (267398233) splicing reads. 593436 splicing junctions. 213145 known splicing junctions. 380291 novel splicing junctions.
sampling 90% (283127541) splicing reads. 598915 splicing junctions. 213526 known splicing junctions. 385389 novel splicing junctions.
sampling 95% (298856849) splicing reads. 603452 splicing junctions. 213830 known splicing junctions. 389622 novel splicing junctions.
sampling 100% (314586157) splicing reads. 607162 splicing junctions. 214074 known splicing junctions. 393088 novel splicing junctions.