[W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15910131) splicing reads. 251860 splicing junctions. 170911 known splicing junctions. 80949 novel splicing junctions.
sampling 10% (31820263) splicing reads. 314612 splicing junctions. 183195 known splicing junctions. 131417 novel splicing junctions.
sampling 15% (47730394) splicing reads. 361561 splicing junctions. 190274 known splicing junctions. 171287 novel splicing junctions.
sampling 20% (63640526) splicing reads. 399499 splicing junctions. 195212 known splicing junctions. 204287 novel splicing junctions.
sampling 25% (79550657) splicing reads. 431073 splicing junctions. 198840 known splicing junctions. 232233 novel splicing junctions.
sampling 30% (95460789) splicing reads. 458652 splicing junctions. 201776 known splicing junctions. 256876 novel splicing junctions.
sampling 35% (111370920) splicing reads. 482349 splicing junctions. 204046 known splicing junctions. 278303 novel splicing junctions.
sampling 40% (127281052) splicing reads. 503677 splicing junctions. 205913 known splicing junctions. 297764 novel splicing junctions.
sampling 45% (143191183) splicing reads. 522606 splicing junctions. 207461 known splicing junctions. 315145 novel splicing junctions.
sampling 50% (159101315) splicing reads. 539254 splicing junctions. 208794 known splicing junctions. 330460 novel splicing junctions.
sampling 55% (175011446) splicing reads. 554292 splicing junctions. 209956 known splicing junctions. 344336 novel splicing junctions.
sampling 60% (190921578) splicing reads. 567691 splicing junctions. 210937 known splicing junctions. 356754 novel splicing junctions.
sampling 65% (206831709) splicing reads. 579689 splicing junctions. 211813 known splicing junctions. 367876 novel splicing junctions.
sampling 70% (222741841) splicing reads. 590146 splicing junctions. 212540 known splicing junctions. 377606 novel splicing junctions.
sampling 75% (238651972) splicing reads. 599630 splicing junctions. 213200 known splicing junctions. 386430 novel splicing junctions.
sampling 80% (254562104) splicing reads. 607779 splicing junctions. 213766 known splicing junctions. 394013 novel splicing junctions.
sampling 85% (270472235) splicing reads. 614970 splicing junctions. 214282 known splicing junctions. 400688 novel splicing junctions.
sampling 90% (286382367) splicing reads. 621193 splicing junctions. 214720 known splicing junctions. 406473 novel splicing junctions.
sampling 95% (302292498) splicing reads. 626139 splicing junctions. 215031 known splicing junctions. 411108 novel splicing junctions.
sampling 100% (318202630) splicing reads. 630276 splicing junctions. 215316 known splicing junctions. 414960 novel splicing junctions.