.command.out - Workdir - NFCORE_RNAFUSION:RNAFUSION:RNA_CONTAMINATION_DETECTION_WORKFLOW:NGSCheckMATE (BT-20_FFPE_RNA_0001_B23LG7FLT4_2)

File Info

Filename: .command.out
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/8a/b945d11610df199f9d44839d70a31c/.command.out
Size: 459 bytes
Task: NFCORE_RNAFUSION:RNAFUSION:RNA_CONTAMINATION_DETECTION_WORKFLOW:NGSCheckMATE (BT-20_FFPE_RNA_0001_B23LG7FLT4_2)
Attempt

Content

Set the path to your reference file with the NCM_REF environment variable
eg. export NCM_REF=/<path>/<to>/<reference>

1.14
samtools mpileup -uf $NCM_REF -l /usr/local/NGSCheckMate/SNP/SNP_GRCh38_hg38_wChr.bed BT-20_FFPE_RNA_0001_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam | bcftools call -c > ./BT-20_FFPE_RNA_0001_B23LG7FLT4_2.Aligned.sortedByCoord.out.vcf
Generate Data Set from .
using this bed file : /usr/local/NGSCheckMate/SNP/SNP_GRCh38_hg38_wChr.bed