File Info

Filename
.command.sh
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d6/82d89fa926bab2cc4c10d3360cd3ca/.command.sh
Size
1.3 KB
Attempt
#!/bin/bash -euo pipefail
python -u /usr/local/src/CTAT-SPLICING/STAR_to_cancer_introns.py \
    --SJ_tab_file BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1.SJ.out.tab \
    --chimJ_file BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1.Chimeric.out.junction \
    --bam_file BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam \
    --output_prefix BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1 \
    --ctat_genome_lib ctat_genome_lib_build_dir \
    --vis --sample_name BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1

# Sort output files to ensure consistent ordering across runs while preserving headers
if [ -f BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1.cancer.introns ]; then
    # Extract header (first line) and sort data lines by fourth column (uniq_mapped) descending, then by first column (intron)
    head -n 1 BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1.cancer.introns > BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1.cancer.introns.tmp
    tail -n +2 BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1.cancer.introns | LC_ALL=C sort -k4,4nr -k1,1 >> BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1.cancer.introns.tmp
    mv BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1.cancer.introns.tmp BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1.cancer.introns
fi

cat <<-END_VERSIONS > versions.yml
"NFCORE_RNAFUSION:RNAFUSION:CTATSPLICING_STARTOCANCERINTRONS":
    ctat-splicing: 0.0.3
END_VERSIONS