Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/56/13a0da7a90913d31925104f03d721b/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/93/e0cca821e550039347ec2df1d82643/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7d/8eadecd22fbd1e2e1a2f97efa0369e/BT-20_FFPE_RNA_0001_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/93/e0cca821e550039347ec2df1d82643/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4b/3e11597d12ae7372784ca3e91eb84f/BT-20_FFPE_RNA_0001_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: BT-20_FFPE_RNA_0001_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (1819478) splicing reads. 145686 splicing junctions. 132105 known splicing junctions. 13581 novel splicing junctions.
sampling 10% (3638957) splicing reads. 167877 splicing junctions. 145972 known splicing junctions. 21905 novel splicing junctions.
sampling 15% (5458436) splicing reads. 180288 splicing junctions. 152265 known splicing junctions. 28023 novel splicing junctions.
sampling 20% (7277915) splicing reads. 188813 splicing junctions. 156017 known splicing junctions. 32796 novel splicing junctions.
sampling 25% (9097394) splicing reads. 195249 splicing junctions. 158511 known splicing junctions. 36738 novel splicing junctions.
sampling 30% (10916873) splicing reads. 200432 splicing junctions. 160284 known splicing junctions. 40148 novel splicing junctions.
sampling 35% (12736351) splicing reads. 204556 splicing junctions. 161614 known splicing junctions. 42942 novel splicing junctions.
sampling 40% (14555830) splicing reads. 208083 splicing junctions. 162715 known splicing junctions. 45368 novel splicing junctions.
sampling 45% (16375309) splicing reads. 211061 splicing junctions. 163584 known splicing junctions. 47477 novel splicing junctions.
sampling 50% (18194788) splicing reads. 213545 splicing junctions. 164278 known splicing junctions. 49267 novel splicing junctions.
sampling 55% (20014267) splicing reads. 215737 splicing junctions. 164820 known splicing junctions. 50917 novel splicing junctions.
sampling 60% (21833746) splicing reads. 217539 splicing junctions. 165275 known splicing junctions. 52264 novel splicing junctions.
sampling 65% (23653225) splicing reads. 219164 splicing junctions. 165701 known splicing junctions. 53463 novel splicing junctions.
sampling 70% (25472703) splicing reads. 220482 splicing junctions. 166033 known splicing junctions. 54449 novel splicing junctions.
sampling 75% (27292182) splicing reads. 221555 splicing junctions. 166287 known splicing junctions. 55268 novel splicing junctions.
sampling 80% (29111661) splicing reads. 222465 splicing junctions. 166505 known splicing junctions. 55960 novel splicing junctions.
sampling 85% (30931140) splicing reads. 223156 splicing junctions. 166641 known splicing junctions. 56515 novel splicing junctions.
sampling 90% (32750619) splicing reads. 223647 splicing junctions. 166740 known splicing junctions. 56907 novel splicing junctions.
sampling 95% (34570098) splicing reads. 223954 splicing junctions. 166810 known splicing junctions. 57144 novel splicing junctions.
sampling 100% (36389577) splicing reads. 224079 splicing junctions. 166838 known splicing junctions. 57241 novel splicing junctions.
null device 
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