[W::hts_idx_load3] The index file is older than the data file: A549_FFPE_01_RNA_0001_B23LG7FLT4_3.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (46) splicing reads. 25 splicing junctions. 6 known splicing junctions. 19 novel splicing junctions.
sampling 10% (92) splicing reads. 38 splicing junctions. 8 known splicing junctions. 30 novel splicing junctions.
sampling 15% (138) splicing reads. 46 splicing junctions. 9 known splicing junctions. 37 novel splicing junctions.
sampling 20% (184) splicing reads. 55 splicing junctions. 11 known splicing junctions. 44 novel splicing junctions.
sampling 25% (230) splicing reads. 61 splicing junctions. 12 known splicing junctions. 49 novel splicing junctions.
sampling 30% (276) splicing reads. 67 splicing junctions. 12 known splicing junctions. 55 novel splicing junctions.
sampling 35% (323) splicing reads. 71 splicing junctions. 12 known splicing junctions. 59 novel splicing junctions.
sampling 40% (369) splicing reads. 77 splicing junctions. 12 known splicing junctions. 65 novel splicing junctions.
sampling 45% (415) splicing reads. 83 splicing junctions. 13 known splicing junctions. 70 novel splicing junctions.
sampling 50% (461) splicing reads. 89 splicing junctions. 13 known splicing junctions. 76 novel splicing junctions.
sampling 55% (507) splicing reads. 92 splicing junctions. 13 known splicing junctions. 79 novel splicing junctions.
sampling 60% (553) splicing reads. 93 splicing junctions. 13 known splicing junctions. 80 novel splicing junctions.
sampling 65% (599) splicing reads. 93 splicing junctions. 13 known splicing junctions. 80 novel splicing junctions.
sampling 70% (646) splicing reads. 96 splicing junctions. 13 known splicing junctions. 83 novel splicing junctions.
sampling 75% (692) splicing reads. 99 splicing junctions. 13 known splicing junctions. 86 novel splicing junctions.
sampling 80% (738) splicing reads. 99 splicing junctions. 13 known splicing junctions. 86 novel splicing junctions.
sampling 85% (784) splicing reads. 102 splicing junctions. 13 known splicing junctions. 89 novel splicing junctions.
sampling 90% (830) splicing reads. 103 splicing junctions. 13 known splicing junctions. 90 novel splicing junctions.
sampling 95% (876) splicing reads. 105 splicing junctions. 13 known splicing junctions. 92 novel splicing junctions.
sampling 100% (923) splicing reads. 105 splicing junctions. 13 known splicing junctions. 92 novel splicing junctions.