File Info

Filename
.command.sh
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4e/9f83728b8552b29789d5e13de872f8/.command.sh
Size
1.3 KB
Attempt
#!/bin/bash -euo pipefail
python -u /usr/local/src/CTAT-SPLICING/STAR_to_cancer_introns.py \
    --SJ_tab_file BreastNB_FFPE_L03_RNA_01_B23LG7FLT4_1.SJ.out.tab \
    --chimJ_file BreastNB_FFPE_L03_RNA_01_B23LG7FLT4_1.Chimeric.out.junction \
    --bam_file BreastNB_FFPE_L03_RNA_01_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam \
    --output_prefix BreastNB_FFPE_L03_RNA_01_B23LG7FLT4_1 \
    --ctat_genome_lib ctat_genome_lib_build_dir \
    --vis --sample_name BreastNB_FFPE_L03_RNA_01_B23LG7FLT4_1

# Sort output files to ensure consistent ordering across runs while preserving headers
if [ -f BreastNB_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns ]; then
    # Extract header (first line) and sort data lines by fourth column (uniq_mapped) descending, then by first column (intron)
    head -n 1 BreastNB_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns > BreastNB_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns.tmp
    tail -n +2 BreastNB_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns | LC_ALL=C sort -k4,4nr -k1,1 >> BreastNB_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns.tmp
    mv BreastNB_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns.tmp BreastNB_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns
fi

cat <<-END_VERSIONS > versions.yml
"NFCORE_RNAFUSION:RNAFUSION:CTATSPLICING_STARTOCANCERINTRONS":
    ctat-splicing: 0.0.3
END_VERSIONS