Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2f/6d3a4c95cdb2aaa2704cfa8b3f82b9/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cf/817c5f6743d444fb0b250eccee3604/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Chimeric.out.junction Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f2/8ac643f08f41ffcd20c58351ba0eb5/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam.bai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2f/6d3a4c95cdb2aaa2704cfa8b3f82b9/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cf/817c5f6743d444fb0b250eccee3604/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.SJ.out.tab Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cf/817c5f6743d444fb0b250eccee3604/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam ==> STAGING COMPLETE (7 inputs) 09:32:35 : 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[21430000], rate=20409523.81/min [21440000], rate=20419047.62/min [21450000], rate=20428571.43/min [21460000], rate=20438095.24/min [21470000], rate=20447619.05/min [21480000], rate=20457142.86/min [21490000], rate=20466666.67/min [21500000], rate=20476190.48/min [21510000], rate=20165625.00/min [21520000], rate=20175000.00/min [21530000], rate=20184375.00/min [21540000], rate=20193750.00/min [21550000], rate=20203125.00/min [21560000], rate=20212500.00/min [21570000], rate=20221875.00/min [21580000], rate=20231250.00/min [21590000], rate=20240625.00/min [21600000], rate=20250000.00/min [21610000], rate=20259375.00/min [21620000], rate=20268750.00/min [21630000], rate=20278125.00/min [21640000], rate=20287500.00/min [21650000], rate=20296875.00/min [21660000], rate=20306250.00/min [21670000], rate=20315625.00/min [21680000], rate=20325000.00/min [21690000], rate=20334375.00/min [21700000], rate=20343750.00/min [21710000], rate=20353125.00/min [21720000], rate=20362500.00/min [21730000], rate=20371875.00/min [21740000], rate=20381250.00/min [21750000], rate=20390625.00/min [21760000], rate=20400000.00/min [21770000], rate=20409375.00/min [21780000], rate=20418750.00/min [21790000], rate=20113846.15/min [21800000], rate=20123076.92/min [21810000], rate=20132307.69/min [21820000], rate=20141538.46/min [21830000], rate=20150769.23/min [21840000], rate=20160000.00/min [21850000], rate=20169230.77/min [21860000], rate=20178461.54/min [21870000], rate=20187692.31/min [21880000], rate=20196923.08/min [21890000], rate=20206153.85/min [21900000], rate=20215384.62/min [21910000], rate=20224615.38/min [21920000], rate=20233846.15/min [21930000], rate=20243076.92/min [21940000], rate=20252307.69/min [21950000], rate=20261538.46/min [21960000], rate=20270769.23/min [21970000], rate=20280000.00/min [21980000], rate=20289230.77/min [21990000], rate=20298461.54/min [22000000], rate=20307692.31/min [22010000], rate=20316923.08/min [22020000], rate=20326153.85/min [22030000], rate=20335384.62/min [22040000], rate=20344615.38/min [22050000], rate=20353846.15/min [22060000], rate=20363076.92/min [22070000], rate=20063636.36/min [22080000], rate=20072727.27/min [22090000], rate=20081818.18/min [22100000], rate=20090909.09/min [22110000], rate=20100000.00/min [22120000], rate=20109090.91/min [22130000], rate=20118181.82/min [22140000], rate=20127272.73/min [22150000], rate=20136363.64/min [22160000], rate=20145454.55/min [22170000], rate=20154545.45/min [22180000], rate=20163636.36/min [22190000], rate=20172727.27/min [22200000], rate=20181818.18/min [22210000], rate=20190909.09/min [22220000], rate=20200000.00/min [22230000], rate=20209090.91/min [22240000], rate=20218181.82/min [22250000], rate=20227272.73/min [22260000], rate=20236363.64/min [22270000], rate=20245454.55/min [22280000], rate=20254545.45/min [22290000], rate=20263636.36/min [22300000], rate=20272727.27/min [22310000], rate=20281818.18/min [22320000], rate=20290909.09/min [22330000], rate=20300000.00/min [22340000], rate=20005970.15/min [22350000], rate=20014925.37/min [22360000], rate=20023880.60/min [22370000], rate=20032835.82/min [22380000], rate=20041791.04/min [22390000], rate=20050746.27/min [22400000], rate=20059701.49/min [22410000], rate=20068656.72/min [22420000], rate=20077611.94/min [22430000], rate=20086567.16/min [22440000], rate=20095522.39/min [22450000], rate=20104477.61/min [22460000], rate=20113432.84/min [22470000], rate=20122388.06/min [22480000], rate=20131343.28/min [22490000], rate=20140298.51/min [22500000], rate=20149253.73/min [22510000], rate=20158208.96/min [22520000], rate=20167164.18/min 09:33:49 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.prelim Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18. -ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns: identified 597 cancer introns 09:33:49 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.prelim 09:33:49 : INFO : Running: touch /tmp/nxf.QqqpZUM8Gj/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/prelim_introns.ok 09:33:49 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.prelim --min_total_reads 5 > ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns 09:33:50 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.prelim --min_total_reads 5 > ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns 09:33:50 : INFO : Running: touch /tmp/nxf.QqqpZUM8Gj/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/introns_filtered.ok 09:33:50 : INFO : -found 453 cancer introns 09:33:50 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns --cancer_introns ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed 09:33:50 : INFO : Creating the BED File. 09:33:52 : INFO : Saving Bed File as ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed 09:33:52 : INFO : Execution Time = 0.04 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns --cancer_introns ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed 09:33:52 : INFO : Running: touch /tmp/nxf.QqqpZUM8Gj/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/intron_igv_bed.ok 09:33:52 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed --bam ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam --output_prefix ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2 [W::hts_idx_load3] The index file is older than the data file: ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam.bai 09:57:34 : INFO : Execution Time = 23.69 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed --bam ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam --output_prefix ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2 09:57:34 : INFO : Running: touch /tmp/nxf.QqqpZUM8Gj/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/reads_alignments_extracted.ok 09:57:34 : INFO : Running: samtools sort -o ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer_intron_reads.sorted.bam ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer_intron_reads.bam 09:57:36 : INFO : Execution Time = 0.04 minutes. CMD: samtools sort -o ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer_intron_reads.sorted.bam ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer_intron_reads.bam 09:57:36 : INFO : Running: touch /tmp/nxf.QqqpZUM8Gj/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/sort_cancer_intron_reads.ok 09:57:36 : INFO : Running: samtools sort -o ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.bam ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.bam [bam_sort_core] merging from 105 files and 1 in-memory blocks... 10:25:43 : INFO : Execution Time = 28.11 minutes. CMD: samtools sort -o ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.bam ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.bam 10:25:43 : INFO : Running: touch /tmp/nxf.QqqpZUM8Gj/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/sort_gene_reads.ok 10:25:43 : INFO : Running: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.sifted.bam.tmp --keep_secondary ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.bam Done with chr chr1. Done with chr chr2. Done with chr chr3. Done with chr chr4. Done with chr chr5. Done with chr chr6. Done with chr chr7. Done with chr chr8. Done with chr chr9. Done with chr chr10. Done with chr chr11. Done with chr chr12. Done with chr chr13. Done with chr chr14. Done with chr chr15. Done with chr chr16. Done with chr chr17. Done with chr chr18. Done with chr chr19. Done with chr chr20. Done with chr chr21. Done with chr chr22. 10:30:10 : INFO : Execution Time = 4.46 minutes. CMD: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.sifted.bam.tmp --keep_secondary ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.bam 10:30:10 : INFO : Running: touch /tmp/nxf.QqqpZUM8Gj/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.sifted.bam.tmp.ok 10:30:10 : INFO : Running: samtools sort -o ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.sifted.bam ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.sifted.bam.tmp 10:30:18 : INFO : Execution Time = 0.13 minutes. CMD: samtools sort -o ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.sifted.bam ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.sifted.bam.tmp 10:30:18 : INFO : Running: touch /tmp/nxf.QqqpZUM8Gj/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.sifted.bam.tmpsorted.ok 10:30:18 : INFO : Running: samtools index ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.sifted.bam 10:30:20 : INFO : Execution Time = 0.02 minutes. CMD: samtools index ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.sifted.bam 10:30:20 : INFO : Running: touch /tmp/nxf.QqqpZUM8Gj/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.gene_reads.sorted.sifted.bam.indexed.ok 10:30:20 : INFO : Running: samtools index ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer_intron_reads.sorted.bam 10:30:20 : INFO : Execution Time = 0.01 minutes. CMD: samtools index ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer_intron_reads.sorted.bam 10:30:20 : INFO : Running: touch /tmp/nxf.QqqpZUM8Gj/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer_intron_reads.sorted.bam.indexed.ok 10:30:20 : INFO : Running: create_report ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.ctat-splicing.igv.html --track-config ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2' 10:31:11 : INFO : Execution Time = 0.85 minutes. CMD: create_report ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.ctat-splicing.igv.html --track-config ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2' 10:31:11 : INFO : Running: touch /tmp/nxf.QqqpZUM8Gj/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/igv_create_html.ok 10:31:11 : INFO : done.