#!/bin/bash -euo pipefail python -u /usr/local/src/CTAT-SPLICING/STAR_to_cancer_introns.py \ --SJ_tab_file ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.SJ.out.tab \ --chimJ_file ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Chimeric.out.junction \ --bam_file ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam \ --output_prefix ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2 \ --ctat_genome_lib ctat_genome_lib_build_dir \ --vis --sample_name ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2 # Sort output files to ensure consistent ordering across runs while preserving headers if [ -f ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns ]; then # Extract header (first line) and sort data lines by fourth column (uniq_mapped) descending, then by first column (intron) head -n 1 ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns > ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.tmp tail -n +2 ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns | LC_ALL=C sort -k4,4nr -k1,1 >> ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.tmp mv ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.tmp ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns fi cat <<-END_VERSIONS > versions.yml "NFCORE_RNAFUSION:RNAFUSION:CTATSPLICING_STARTOCANCERINTRONS": ctat-splicing: 0.0.3 END_VERSIONS