Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/40/ced43a1f7316b12a1707c3ba78fa86/.command.sh
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cf/817c5f6743d444fb0b250eccee3604/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Chimeric.out.junction
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f2/8ac643f08f41ffcd20c58351ba0eb5/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam.bai
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/40/ced43a1f7316b12a1707c3ba78fa86/.command.run
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cf/817c5f6743d444fb0b250eccee3604/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.SJ.out.tab
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cf/817c5f6743d444fb0b250eccee3604/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam
==> STAGING COMPLETE (7 inputs)
09:12:44 : INFO : -reading targets list: ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-mapping reads to genes
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[22520000], rate=19870588.24/min 09:13:59 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.prelim
Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18.
-ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns: identified 597 cancer introns
09:13:59 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.prelim
09:13:59 : INFO : Running: touch /tmp/nxf.L8Cmvsq7H1/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/prelim_introns.ok
09:13:59 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.prelim --min_total_reads 5 > ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns
09:13:59 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.prelim --min_total_reads 5 > ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns
09:13:59 : INFO : Running: touch /tmp/nxf.L8Cmvsq7H1/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/introns_filtered.ok
09:13:59 : INFO : -found 453 cancer introns
09:13:59 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns --cancer_introns ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed
09:14:00 : INFO : Creating the BED File.
09:14:01 : INFO : Saving Bed File as ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed
09:14:01 : INFO : Execution Time = 0.03 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns --cancer_introns ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed
09:14:01 : INFO : Running: touch /tmp/nxf.L8Cmvsq7H1/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/intron_igv_bed.ok
09:14:01 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed --bam ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam --output_prefix ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2
[W::hts_idx_load3] The index file is older than the data file: ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam.bai