Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/40/ced43a1f7316b12a1707c3ba78fa86/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cf/817c5f6743d444fb0b250eccee3604/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Chimeric.out.junction Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f2/8ac643f08f41ffcd20c58351ba0eb5/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam.bai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/40/ced43a1f7316b12a1707c3ba78fa86/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cf/817c5f6743d444fb0b250eccee3604/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.SJ.out.tab Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cf/817c5f6743d444fb0b250eccee3604/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam ==> STAGING COMPLETE (7 inputs) 09:12:44 : 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rate=19808955.22/min [22130000], rate=19817910.45/min [22140000], rate=19826865.67/min [22150000], rate=19835820.90/min [22160000], rate=19844776.12/min [22170000], rate=19853731.34/min [22180000], rate=19862686.57/min [22190000], rate=19871641.79/min [22200000], rate=19880597.01/min [22210000], rate=19889552.24/min [22220000], rate=19898507.46/min [22230000], rate=19907462.69/min [22240000], rate=19916417.91/min [22250000], rate=19925373.13/min [22260000], rate=19934328.36/min [22270000], rate=19943283.58/min [22280000], rate=19952238.81/min [22290000], rate=19961194.03/min [22300000], rate=19970149.25/min [22310000], rate=19979104.48/min [22320000], rate=19988059.70/min [22330000], rate=19997014.93/min [22340000], rate=20005970.15/min [22350000], rate=20014925.37/min [22360000], rate=20023880.60/min [22370000], rate=20032835.82/min [22380000], rate=20041791.04/min [22390000], rate=20050746.27/min [22400000], rate=20059701.49/min [22410000], rate=20068656.72/min [22420000], rate=20077611.94/min [22430000], rate=20086567.16/min [22440000], rate=20095522.39/min [22450000], rate=20104477.61/min [22460000], rate=20113432.84/min [22470000], rate=20122388.06/min [22480000], rate=20131343.28/min [22490000], rate=20140298.51/min [22500000], rate=20149253.73/min [22510000], rate=19861764.71/min [22520000], rate=19870588.24/min 09:13:59 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.prelim Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18. -ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns: identified 597 cancer introns 09:13:59 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.prelim 09:13:59 : INFO : Running: touch /tmp/nxf.L8Cmvsq7H1/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/prelim_introns.ok 09:13:59 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.prelim --min_total_reads 5 > ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns 09:13:59 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns.prelim --min_total_reads 5 > ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns 09:13:59 : INFO : Running: touch /tmp/nxf.L8Cmvsq7H1/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/introns_filtered.ok 09:13:59 : INFO : -found 453 cancer introns 09:13:59 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns --cancer_introns ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed 09:14:00 : INFO : Creating the BED File. 09:14:01 : INFO : Saving Bed File as ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed 09:14:01 : INFO : Execution Time = 0.03 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns --cancer_introns ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed 09:14:01 : INFO : Running: touch /tmp/nxf.L8Cmvsq7H1/ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.chckpts/intron_igv_bed.ok 09:14:01 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.introns.for_IGV.bed --bam ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam --output_prefix ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2 [W::hts_idx_load3] The index file is older than the data file: ColoN_FFPE_L01_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam.bai