07:31:25 : INFO : -reading targets list: ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-mapping reads to genes
[300000], rate=18000000.00/min
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[2780000], rate=20850000.00/min
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[2800000], rate=18666666.67/min
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[2820000], rate=18800000.00/min
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[2850000], rate=19000000.00/min
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[2900000], rate=19333333.33/min
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[2940000], rate=19600000.00/min
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[2970000], rate=19800000.00/min
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[2990000], rate=19933333.33/min
[3000000], rate=20000000.00/min
[3010000], rate=20066666.67/min
[3020000], rate=20133333.33/min
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[3050000], rate=20333333.33/min
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[3100000], rate=20666666.67/min
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[3150000], rate=21000000.00/min 07:31:41 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns.prelim
Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18.
-BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.introns: identified 110 cancer introns
07:31:41 : INFO : Execution Time = 0.00 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns.prelim
07:31:41 : INFO : Running: touch /tmp/nxf.awXffl4axk/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.chckpts/prelim_introns.ok
07:31:41 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns.prelim --min_total_reads 5 > BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns
07:31:41 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns.prelim --min_total_reads 5 > BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns
07:31:41 : INFO : Running: touch /tmp/nxf.awXffl4axk/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.chckpts/introns_filtered.ok
07:31:41 : INFO : -found 81 cancer introns
07:31:41 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.introns --cancer_introns BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed
07:31:42 : INFO : Creating the BED File.
07:31:42 : INFO : Saving Bed File as BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed
07:31:42 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.introns --cancer_introns BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed
07:31:42 : INFO : Running: touch /tmp/nxf.awXffl4axk/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.chckpts/intron_igv_bed.ok
07:31:42 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed --bam BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam --output_prefix BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1
[W::hts_idx_load3] The index file is older than the data file: BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam.bai
07:32:31 : INFO : Execution Time = 0.81 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed --bam BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam --output_prefix BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1
07:32:31 : INFO : Running: touch /tmp/nxf.awXffl4axk/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.chckpts/reads_alignments_extracted.ok
07:32:31 : INFO : Running: samtools sort -o BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer_intron_reads.sorted.bam BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer_intron_reads.bam
07:32:31 : INFO : Execution Time = 0.00 minutes. CMD: samtools sort -o BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer_intron_reads.sorted.bam BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer_intron_reads.bam
07:32:31 : INFO : Running: touch /tmp/nxf.awXffl4axk/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.chckpts/sort_cancer_intron_reads.ok
07:32:31 : INFO : Running: samtools sort -o BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.bam BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.bam
[bam_sort_core] merging from 4 files and 1 in-memory blocks...
07:33:34 : INFO : Execution Time = 1.05 minutes. CMD: samtools sort -o BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.bam BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.bam
07:33:34 : INFO : Running: touch /tmp/nxf.awXffl4axk/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.chckpts/sort_gene_reads.ok
07:33:34 : INFO : Running: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.tmp --keep_secondary BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.bam
07:33:44 : INFO : Execution Time = 0.16 minutes. CMD: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.tmp --keep_secondary BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.bam
07:33:44 : INFO : Running: touch /tmp/nxf.awXffl4axk/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.chckpts/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.tmp.ok
07:33:44 : INFO : Running: samtools sort -o BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.tmp
07:33:45 : INFO : Execution Time = 0.01 minutes. CMD: samtools sort -o BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.tmp
07:33:45 : INFO : Running: touch /tmp/nxf.awXffl4axk/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.chckpts/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.tmpsorted.ok
07:33:45 : INFO : Running: samtools index BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam
07:33:45 : INFO : Execution Time = 0.00 minutes. CMD: samtools index BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam
07:33:45 : INFO : Running: touch /tmp/nxf.awXffl4axk/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.chckpts/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.gene_reads.sorted.sifted.bam.indexed.ok
07:33:45 : INFO : Running: samtools index BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer_intron_reads.sorted.bam
07:33:45 : INFO : Execution Time = 0.00 minutes. CMD: samtools index BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer_intron_reads.sorted.bam
07:33:45 : INFO : Running: touch /tmp/nxf.awXffl4axk/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.chckpts/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.cancer_intron_reads.sorted.bam.indexed.ok
07:33:45 : INFO : Running: create_report BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.ctat-splicing.igv.html --track-config BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1'
07:33:53 : INFO : Execution Time = 0.14 minutes. CMD: create_report BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.ctat-splicing.igv.html --track-config BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1'
07:33:53 : INFO : Running: touch /tmp/nxf.awXffl4axk/BreastNP_FFPE_L03_RNA_01_B23LG7FLT4_1.chckpts/igv_create_html.ok
07:33:53 : INFO : done.