FastQCFastQC Report
Sat 16 May 2026
EndoN_FFPE_L03_RNA_01_B23LG7FLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEndoN_FFPE_L03_RNA_01_B23LG7FLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113065956
Total Bases17 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACAAGAGGATCTGGG27532072.435045081120616TruSeq Adapter, Index 4 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACAAGAGGATCTGGT16811591.4868834611896795TruSeq Adapter, Index 4 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACAAGAGGATCTAGT10211140.9031135773530276TruSeq Adapter, Index 4 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACAAGAGGATCTAGG8954810.7919987869735077TruSeq Adapter, Index 4 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)