FastQCFastQC Report
Sat 16 May 2026
SkinN_FFPE_L01_RNA_01_B23LG7FLT4_2_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSkinN_FFPE_L01_RNA_01_B23LG7FLT4_2_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115367823
Total Bases17.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCATGTCGATCTGGG46888044.064221615761961TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCATGTCGATCTGGT9568500.8293907045467955TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCATGTCGATCTAGG5433630.47098314406088776TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCATGTCGATCTAGT2011050.17431636895844TruSeq Adapter, Index 9 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)