Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44393639723(97.9992%) Q30 bases: 42726471226(94.3189%) Read2 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44346391912(97.8949%) Q30 bases: 42368688879(93.5291%) Read1 after filtering: total reads: 295131003 total bases: 37649126663 Q20 bases: 37454019542(99.4818%) Q30 bases: 36629553220(97.2919%) Read2 after filtering: total reads: 295131003 total bases: 37690315782 Q20 bases: 37381208795(99.1799%) Q30 bases: 36344283836(96.4287%) Filtering result: reads passed filter: 590262006 reads failed due to low quality: 8020850 reads failed due to too many N: 209352 reads failed due to too short: 1507792 reads with adapter trimmed: 351219202 bases trimmed due to adapters: 13938489238 Duplication rate: 24.8973% Insert size peak (evaluated by paired-end reads): 119 JSON report: 786-O_FFPE_RNA_0001_B23LG7FLT4_2.fastp.json HTML report: 786-O_FFPE_RNA_0001_B23LG7FLT4_2.fastp.html fastp --in1 786-O_FFPE_RNA_0001_B23LG7FLT4_2_1.fastq.gz --in2 786-O_FFPE_RNA_0001_B23LG7FLT4_2_2.fastq.gz --out1 786-O_FFPE_RNA_0001_B23LG7FLT4_2_1.fastp.fastq.gz --out2 786-O_FFPE_RNA_0001_B23LG7FLT4_2_2.fastp.fastq.gz --json 786-O_FFPE_RNA_0001_B23LG7FLT4_2.fastp.json --html 786-O_FFPE_RNA_0001_B23LG7FLT4_2.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 757 seconds