Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44319711981(97.836%) Q30 bases: 42619471199(94.0827%) Read2 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44277614812(97.7431%) Q30 bases: 42260162630(93.2895%) Read1 after filtering: total reads: 294893040 total bases: 37425087669 Q20 bases: 37207663328(99.419%) Q30 bases: 36318576392(97.0434%) Read2 after filtering: total reads: 294893040 total bases: 37469142096 Q20 bases: 37157029825(99.167%) Q30 bases: 36113551692(96.3821%) Filtering result: reads passed filter: 589786080 reads failed due to low quality: 8404944 reads failed due to too many N: 201184 reads failed due to too short: 1607792 reads with adapter trimmed: 357781866 bases trimmed due to adapters: 14325019652 Duplication rate: 24.3127% Insert size peak (evaluated by paired-end reads): 119 JSON report: 786-O_FFPE_RNA_0001_B23LG7FLT4_3.fastp.json HTML report: 786-O_FFPE_RNA_0001_B23LG7FLT4_3.fastp.html fastp --in1 786-O_FFPE_RNA_0001_B23LG7FLT4_3_1.fastq.gz --in2 786-O_FFPE_RNA_0001_B23LG7FLT4_3_2.fastq.gz --out1 786-O_FFPE_RNA_0001_B23LG7FLT4_3_1.fastp.fastq.gz --out2 786-O_FFPE_RNA_0001_B23LG7FLT4_3_2.fastp.fastq.gz --json 786-O_FFPE_RNA_0001_B23LG7FLT4_3.fastp.json --html 786-O_FFPE_RNA_0001_B23LG7FLT4_3.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 773 seconds