Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44362226040(97.9299%) Q30 bases: 42761422163(94.3961%) Read2 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44363270235(97.9322%) Q30 bases: 42409980513(93.6203%) Read1 after filtering: total reads: 295260444 total bases: 37480695317 Q20 bases: 37284567701(99.4767%) Q30 bases: 36460987872(97.2794%) Read2 after filtering: total reads: 295260444 total bases: 37523067776 Q20 bases: 37225243302(99.2063%) Q30 bases: 36215482973(96.5153%) Filtering result: reads passed filter: 590520888 reads failed due to low quality: 7856880 reads failed due to too many N: 202266 reads failed due to too short: 1419966 reads with adapter trimmed: 357898898 bases trimmed due to adapters: 14313428567 Duplication rate: 24.9729% Insert size peak (evaluated by paired-end reads): 118 JSON report: A673_FFPE_RNA_0001_B23LG7FLT4_1.fastp.json HTML report: A673_FFPE_RNA_0001_B23LG7FLT4_1.fastp.html fastp --in1 A673_FFPE_RNA_0001_B23LG7FLT4_1_1.fastq.gz --in2 A673_FFPE_RNA_0001_B23LG7FLT4_1_2.fastq.gz --out1 A673_FFPE_RNA_0001_B23LG7FLT4_1_1.fastp.fastq.gz --out2 A673_FFPE_RNA_0001_B23LG7FLT4_1_2.fastp.fastq.gz --json A673_FFPE_RNA_0001_B23LG7FLT4_1.fastp.json --html A673_FFPE_RNA_0001_B23LG7FLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 762 seconds