Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44508706955(98.2532%) Q30 bases: 42930776655(94.7699%) Read2 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44313687809(97.8227%) Q30 bases: 42295993589(93.3686%) Read1 after filtering: total reads: 295167076 total bases: 37642078406 Q20 bases: 37447184259(99.4822%) Q30 bases: 36617868051(97.2791%) Read2 after filtering: total reads: 295167076 total bases: 37686700426 Q20 bases: 37382859657(99.1938%) Q30 bases: 36358713640(96.4762%) Filtering result: reads passed filter: 590334152 reads failed due to low quality: 8060204 reads failed due to too many N: 193268 reads failed due to too short: 1412376 reads with adapter trimmed: 354160034 bases trimmed due to adapters: 13959202491 Duplication rate: 25.8599% Insert size peak (evaluated by paired-end reads): 119 JSON report: SW780_FFPE_RNA_0001_B23LG7FLT4_2.fastp.json HTML report: SW780_FFPE_RNA_0001_B23LG7FLT4_2.fastp.html fastp --in1 SW780_FFPE_RNA_0001_B23LG7FLT4_2_1.fastq.gz --in2 SW780_FFPE_RNA_0001_B23LG7FLT4_2_2.fastq.gz --out1 SW780_FFPE_RNA_0001_B23LG7FLT4_2_1.fastp.fastq.gz --out2 SW780_FFPE_RNA_0001_B23LG7FLT4_2_2.fastp.fastq.gz --json SW780_FFPE_RNA_0001_B23LG7FLT4_2.fastp.json --html SW780_FFPE_RNA_0001_B23LG7FLT4_2.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 767 seconds