FastQCFastQC Report
Tue 3 Mar 2026
NTC_0001_0001_B23LG7FLT4_2_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23LG7FLT4_2_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173175
Total Bases177.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGT76142164.90259338973298TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGT18687515.928996100326037TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCGGTCACCTCATCATCTATCTAGT406853.467939565708441TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCGGTCACCTCATCATCTATCTGGT183991.5683082234108296TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGG89220.7605003516099473TruSeq Adapter, Index 1 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGTA73180.6237773563193897TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGG69500.5924094870756708TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTCATCATCTATCTAGT43540.3711296268672619TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTCATCATCTATCTAGT41660.35510473714492724TruSeq Adapter, Index 12 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGTATG28330.24148144991156476TruSeq Adapter, Index 1 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGCTCATCATCTATCTAGT27120.23116755812218978TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCGAGTCACCTCATCATCTATCTAGT26590.2266499030408933TruSeq Adapter, Index 12 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGTA25990.2215355765337652TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTCATCATCTATCTGGT22010.1876105440364822TruSeq Adapter, Index 12 (97% over 35bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA21650.18454194813220534No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTCATCATCTATCTGGT21390.1823257399791165TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCGAGTCACCTCATCATCTATCTGGT18830.1605046135487033TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGCTCATCATCTATCTGGT18410.15692458499371364TruSeq Adapter, Index 12 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGTG14410.12282907494619302TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGTG13780.11745903211370853TruSeq Adapter, Index 1 (97% over 36bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG13620.1160952117118077No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC12210.10407654442005669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)