FastQCFastQC Report
Tue 3 Mar 2026
NTC_0001_0001_B23LG7FLT4_2_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23LG7FLT4_2_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147200
Total Bases171.5 Mbp
Sequences flagged as poor quality0
Sequence length31-151
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGT75709465.99494421199442TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGT18437116.071391213389123TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCGGTCACCTCATCATCTATCTAGT398643.47489539748954TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCGGTCACCTCATCATCTATCTGGT177531.5475069735006972TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGG84680.7381450488145049TruSeq Adapter, Index 1 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGTA73020.6365062761506276TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGG64140.5591004184100418TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTCATCATCTATCTAGT41960.3657601115760111TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTCATCATCTATCTAGT39080.3406555090655509TruSeq Adapter, Index 12 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGTATG28320.24686192468619245TruSeq Adapter, Index 1 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGTA25880.22559274755927475TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGCTCATCATCTATCTAGT25180.21949093444909346TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCGAGTCACCTCATCATCTATCTAGT24720.21548117154811716TruSeq Adapter, Index 12 (97% over 35bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA21030.18331589958158997No Hit
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTCATCATCTATCTGGT20770.1810495118549512TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTCATCATCTATCTGGT19820.17276847977684798TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCGAGTCACCTCATCATCTATCTGGT17180.14975592747559274TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGCTCATCATCTATCTGGT16580.1445258019525802TruSeq Adapter, Index 12 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTAGTG14340.125TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCATCATCTATCTGGTG13710.11950836820083682TruSeq Adapter, Index 1 (97% over 36bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG13310.11602161785216178No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC12160.10599721059972106No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)