FastQCFastQC Report
Tue 3 Mar 2026
BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16590658
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCGGACATCTAGT543670732.76968882126315TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCGGACATCTGGT14796908.918814431591562TruSeq Adapter, Index 8 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCGGACATCTAGTA758960.4574622658124831TruSeq Adapter, Index 8 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCGGACATCTGGTA393170.23698276463778595TruSeq Adapter, Index 8 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCGGACATCTAGTATG357010.2151873662877024TruSeq Adapter, Index 8 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCGGACATCTGGG188050.1133469208997015TruSeq Adapter, Index 8 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)