FastQCFastQC Report
Tue 3 Mar 2026
ColoN_FFPE_L03_RNA_01_B23LG7FLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameColoN_FFPE_L03_RNA_01_B23LG7FLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19058536
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC65

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAGT671124235.213838040865255TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTGGT16374518.591693506783523TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAGG3990642.093885910229411TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTGGG3938702.0666330299452174TruSeq Adapter, Index 9 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAGTG646580.33926005649122265TruSeq Adapter, Index 9 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTGGTG378720.19871410899557027TruSeq Adapter, Index 9 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGAACTCATCTAGTA241250.12658369981828616TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)