Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 42654 total bases: 6440754 Q20 bases: 5503848(85.4535%) Q30 bases: 4067124(63.1467%) Read2 before filtering: total reads: 42654 total bases: 6440754 Q20 bases: 6012367(93.3488%) Q30 bases: 4702832(73.0168%) Read1 after filtering: total reads: 1330 total bases: 148558 Q20 bases: 140438(94.5341%) Q30 bases: 126436(85.1088%) Read2 after filtering: total reads: 1330 total bases: 150283 Q20 bases: 147301(98.0157%) Q30 bases: 136209(90.635%) Filtering result: reads passed filter: 2660 reads failed due to low quality: 4588 reads failed due to too many N: 0 reads failed due to too short: 78060 reads with adapter trimmed: 42678 bases trimmed due to adapters: 6168192 Duplication rate: 41.3068% Insert size peak (evaluated by paired-end reads): 36 JSON report: NTC_0001_0001_A23WKFTLT4_1.fastp.json HTML report: NTC_0001_0001_A23WKFTLT4_1.fastp.html fastp --in1 NTC_0001_0001_A23WKFTLT4_1_1.fastq.gz --in2 NTC_0001_0001_A23WKFTLT4_1_2.fastq.gz --out1 NTC_0001_0001_A23WKFTLT4_1_1.fastp.fastq.gz --out2 NTC_0001_0001_A23WKFTLT4_1_2.fastp.fastq.gz --json NTC_0001_0001_A23WKFTLT4_1.fastp.json --html NTC_0001_0001_A23WKFTLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 1 seconds