FastQCFastQC Report
Mon 8 Jun 2026
NTC_0001_0001_A23WKFTLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_A23WKFTLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42654
Total Bases6.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGG3582383.98508932339288TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGT17484.09809162094997TruSeq Adapter, Index 27 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGGG6461.5145121207858583TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTAGG4841.1347118675856895TruSeq Adapter, Index 27 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGGGGG3080.7220893702818024TruSeq Adapter, Index 27 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATGTGGG1430.3352557790594083TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATTTGGG1250.2930557509260562TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTAGT1210.2836779668964224TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCGGGG1100.2578890608149294TruSeq Adapter, Index 27 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGGGTG1020.23913349275566184TruSeq Adapter, Index 27 (97% over 34bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGGG610.14301120645191542TruSeq Adapter, Index 15 (96% over 31bp)
GTCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGGG570.1336334224222816TruSeq Adapter, Index 15 (97% over 35bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGG550.1289445304074647TruSeq Adapter, Index 15 (97% over 36bp)
GNTCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGG530.12425563839264782TruSeq Adapter, Index 15 (97% over 36bp)
GATCGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGGG500.11722230037042247TruSeq Adapter, Index 27 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)