[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_bst_01_03_A23WKFTLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (12498098) splicing reads. 244910 splicing junctions. 176449 known splicing junctions. 68461 novel splicing junctions.
sampling 10% (24996197) splicing reads. 293316 splicing junctions. 187122 known splicing junctions. 106194 novel splicing junctions.
sampling 15% (37494296) splicing reads. 324900 splicing junctions. 192667 known splicing junctions. 132233 novel splicing junctions.
sampling 20% (49992395) splicing reads. 347656 splicing junctions. 196163 known splicing junctions. 151493 novel splicing junctions.
sampling 25% (62490494) splicing reads. 365250 splicing junctions. 198519 known splicing junctions. 166731 novel splicing junctions.
sampling 30% (74988593) splicing reads. 378651 splicing junctions. 200279 known splicing junctions. 178372 novel splicing junctions.
sampling 35% (87486692) splicing reads. 389406 splicing junctions. 201641 known splicing junctions. 187765 novel splicing junctions.
sampling 40% (99984791) splicing reads. 398347 splicing junctions. 202724 known splicing junctions. 195623 novel splicing junctions.
sampling 45% (112482890) splicing reads. 405839 splicing junctions. 203561 known splicing junctions. 202278 novel splicing junctions.
sampling 50% (124980989) splicing reads. 412022 splicing junctions. 204238 known splicing junctions. 207784 novel splicing junctions.
sampling 55% (137479087) splicing reads. 417240 splicing junctions. 204800 known splicing junctions. 212440 novel splicing junctions.
sampling 60% (149977186) splicing reads. 421630 splicing junctions. 205252 known splicing junctions. 216378 novel splicing junctions.
sampling 65% (162475285) splicing reads. 425329 splicing junctions. 205632 known splicing junctions. 219697 novel splicing junctions.
sampling 70% (174973384) splicing reads. 428528 splicing junctions. 205975 known splicing junctions. 222553 novel splicing junctions.
sampling 75% (187471483) splicing reads. 431033 splicing junctions. 206226 known splicing junctions. 224807 novel splicing junctions.
sampling 80% (199969582) splicing reads. 433059 splicing junctions. 206406 known splicing junctions. 226653 novel splicing junctions.
sampling 85% (212467681) splicing reads. 434674 splicing junctions. 206573 known splicing junctions. 228101 novel splicing junctions.
sampling 90% (224965780) splicing reads. 435862 splicing junctions. 206676 known splicing junctions. 229186 novel splicing junctions.
sampling 95% (237463879) splicing reads. 436706 splicing junctions. 206757 known splicing junctions. 229949 novel splicing junctions.
sampling 100% (249961978) splicing reads. 437195 splicing junctions. 206810 known splicing junctions. 230385 novel splicing junctions.