[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_bst_01_02_A23WKFTLT4_3.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (7572427) splicing reads. 205291 splicing junctions. 162833 known splicing junctions. 42458 novel splicing junctions.
sampling 10% (15144855) splicing reads. 234225 splicing junctions. 172190 known splicing junctions. 62035 novel splicing junctions.
sampling 15% (22717283) splicing reads. 250530 splicing junctions. 176400 known splicing junctions. 74130 novel splicing junctions.
sampling 20% (30289711) splicing reads. 260981 splicing junctions. 178842 known splicing junctions. 82139 novel splicing junctions.
sampling 25% (37862139) splicing reads. 268339 splicing junctions. 180380 known splicing junctions. 87959 novel splicing junctions.
sampling 30% (45434567) splicing reads. 273840 splicing junctions. 181507 known splicing junctions. 92333 novel splicing junctions.
sampling 35% (53006995) splicing reads. 278173 splicing junctions. 182372 known splicing junctions. 95801 novel splicing junctions.
sampling 40% (60579423) splicing reads. 281450 splicing junctions. 182952 known splicing junctions. 98498 novel splicing junctions.
sampling 45% (68151851) splicing reads. 284162 splicing junctions. 183456 known splicing junctions. 100706 novel splicing junctions.
sampling 50% (75724279) splicing reads. 286365 splicing junctions. 183855 known splicing junctions. 102510 novel splicing junctions.
sampling 55% (83296706) splicing reads. 288189 splicing junctions. 184189 known splicing junctions. 104000 novel splicing junctions.
sampling 60% (90869134) splicing reads. 289662 splicing junctions. 184462 known splicing junctions. 105200 novel splicing junctions.
sampling 65% (98441562) splicing reads. 290923 splicing junctions. 184683 known splicing junctions. 106240 novel splicing junctions.
sampling 70% (106013990) splicing reads. 291974 splicing junctions. 184872 known splicing junctions. 107102 novel splicing junctions.
sampling 75% (113586418) splicing reads. 292837 splicing junctions. 185038 known splicing junctions. 107799 novel splicing junctions.
sampling 80% (121158846) splicing reads. 293464 splicing junctions. 185147 known splicing junctions. 108317 novel splicing junctions.
sampling 85% (128731274) splicing reads. 294005 splicing junctions. 185242 known splicing junctions. 108763 novel splicing junctions.
sampling 90% (136303702) splicing reads. 294391 splicing junctions. 185303 known splicing junctions. 109088 novel splicing junctions.
sampling 95% (143876130) splicing reads. 294670 splicing junctions. 185344 known splicing junctions. 109326 novel splicing junctions.
sampling 100% (151448558) splicing reads. 294845 splicing junctions. 185385 known splicing junctions. 109460 novel splicing junctions.