[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_bld_01_A23WKFTLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (6684508) splicing reads. 196481 splicing junctions. 157504 known splicing junctions. 38977 novel splicing junctions.
sampling 10% (13369017) splicing reads. 220911 splicing junctions. 166069 known splicing junctions. 54842 novel splicing junctions.
sampling 15% (20053525) splicing reads. 233831 splicing junctions. 169897 known splicing junctions. 63934 novel splicing junctions.
sampling 20% (26738034) splicing reads. 241869 splicing junctions. 171965 known splicing junctions. 69904 novel splicing junctions.
sampling 25% (33422542) splicing reads. 247286 splicing junctions. 173291 known splicing junctions. 73995 novel splicing junctions.
sampling 30% (40107051) splicing reads. 251267 splicing junctions. 174261 known splicing junctions. 77006 novel splicing junctions.
sampling 35% (46791559) splicing reads. 254273 splicing junctions. 174939 known splicing junctions. 79334 novel splicing junctions.
sampling 40% (53476068) splicing reads. 256781 splicing junctions. 175482 known splicing junctions. 81299 novel splicing junctions.
sampling 45% (60160576) splicing reads. 258673 splicing junctions. 175910 known splicing junctions. 82763 novel splicing junctions.
sampling 50% (66845085) splicing reads. 260218 splicing junctions. 176249 known splicing junctions. 83969 novel splicing junctions.
sampling 55% (73529593) splicing reads. 261542 splicing junctions. 176528 known splicing junctions. 85014 novel splicing junctions.
sampling 60% (80214102) splicing reads. 262678 splicing junctions. 176787 known splicing junctions. 85891 novel splicing junctions.
sampling 65% (86898610) splicing reads. 263583 splicing junctions. 176966 known splicing junctions. 86617 novel splicing junctions.
sampling 70% (93583119) splicing reads. 264370 splicing junctions. 177138 known splicing junctions. 87232 novel splicing junctions.
sampling 75% (100267627) splicing reads. 264951 splicing junctions. 177237 known splicing junctions. 87714 novel splicing junctions.
sampling 80% (106952136) splicing reads. 265532 splicing junctions. 177332 known splicing junctions. 88200 novel splicing junctions.
sampling 85% (113636644) splicing reads. 265953 splicing junctions. 177423 known splicing junctions. 88530 novel splicing junctions.
sampling 90% (120321153) splicing reads. 266218 splicing junctions. 177475 known splicing junctions. 88743 novel splicing junctions.
sampling 95% (127005661) splicing reads. 266425 splicing junctions. 177522 known splicing junctions. 88903 novel splicing junctions.
sampling 100% (133690170) splicing reads. 266538 splicing junctions. 177540 known splicing junctions. 88998 novel splicing junctions.