[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_bst_02_A23WKFTLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16781504) splicing reads. 261228 splicing junctions. 183580 known splicing junctions. 77648 novel splicing junctions.
sampling 10% (33563009) splicing reads. 322291 splicing junctions. 196322 known splicing junctions. 125969 novel splicing junctions.
sampling 15% (50344513) splicing reads. 366106 splicing junctions. 203216 known splicing junctions. 162890 novel splicing junctions.
sampling 20% (67126018) splicing reads. 400750 splicing junctions. 207698 known splicing junctions. 193052 novel splicing junctions.
sampling 25% (83907522) splicing reads. 428823 splicing junctions. 211043 known splicing junctions. 217780 novel splicing junctions.
sampling 30% (100689027) splicing reads. 452332 splicing junctions. 213532 known splicing junctions. 238800 novel splicing junctions.
sampling 35% (117470531) splicing reads. 472537 splicing junctions. 215580 known splicing junctions. 256957 novel splicing junctions.
sampling 40% (134252036) splicing reads. 489861 splicing junctions. 217115 known splicing junctions. 272746 novel splicing junctions.
sampling 45% (151033540) splicing reads. 504962 splicing junctions. 218401 known splicing junctions. 286561 novel splicing junctions.
sampling 50% (167815045) splicing reads. 518208 splicing junctions. 219512 known splicing junctions. 298696 novel splicing junctions.
sampling 55% (184596550) splicing reads. 529751 splicing junctions. 220357 known splicing junctions. 309394 novel splicing junctions.
sampling 60% (201378054) splicing reads. 539835 splicing junctions. 221180 known splicing junctions. 318655 novel splicing junctions.
sampling 65% (218159559) splicing reads. 548544 splicing junctions. 221834 known splicing junctions. 326710 novel splicing junctions.
sampling 70% (234941063) splicing reads. 555946 splicing junctions. 222429 known splicing junctions. 333517 novel splicing junctions.
sampling 75% (251722568) splicing reads. 562579 splicing junctions. 222905 known splicing junctions. 339674 novel splicing junctions.
sampling 80% (268504072) splicing reads. 568028 splicing junctions. 223313 known splicing junctions. 344715 novel splicing junctions.
sampling 85% (285285577) splicing reads. 572625 splicing junctions. 223642 known splicing junctions. 348983 novel splicing junctions.
sampling 90% (302067081) splicing reads. 576380 splicing junctions. 223891 known splicing junctions. 352489 novel splicing junctions.
sampling 95% (318848586) splicing reads. 579347 splicing junctions. 224108 known splicing junctions. 355239 novel splicing junctions.
sampling 100% (335630091) splicing reads. 581504 splicing junctions. 224297 known splicing junctions. 357207 novel splicing junctions.