[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_bst_01_01_A23WKFTLT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10404062) splicing reads. 232395 splicing junctions. 171289 known splicing junctions. 61106 novel splicing junctions.
sampling 10% (20808125) splicing reads. 275796 splicing junctions. 181739 known splicing junctions. 94057 novel splicing junctions.
sampling 15% (31212187) splicing reads. 303130 splicing junctions. 186971 known splicing junctions. 116159 novel splicing junctions.
sampling 20% (41616250) splicing reads. 322306 splicing junctions. 190117 known splicing junctions. 132189 novel splicing junctions.
sampling 25% (52020313) splicing reads. 336824 splicing junctions. 192324 known splicing junctions. 144500 novel splicing junctions.
sampling 30% (62424375) splicing reads. 348187 splicing junctions. 193906 known splicing junctions. 154281 novel splicing junctions.
sampling 35% (72828438) splicing reads. 357202 splicing junctions. 195134 known splicing junctions. 162068 novel splicing junctions.
sampling 40% (83232501) splicing reads. 364466 splicing junctions. 196091 known splicing junctions. 168375 novel splicing junctions.
sampling 45% (93636563) splicing reads. 370369 splicing junctions. 196807 known splicing junctions. 173562 novel splicing junctions.
sampling 50% (104040626) splicing reads. 375378 splicing junctions. 197459 known splicing junctions. 177919 novel splicing junctions.
sampling 55% (114444689) splicing reads. 379594 splicing junctions. 197957 known splicing junctions. 181637 novel splicing junctions.
sampling 60% (124848751) splicing reads. 383147 splicing junctions. 198370 known splicing junctions. 184777 novel splicing junctions.
sampling 65% (135252814) splicing reads. 386055 splicing junctions. 198714 known splicing junctions. 187341 novel splicing junctions.
sampling 70% (145656877) splicing reads. 388400 splicing junctions. 198986 known splicing junctions. 189414 novel splicing junctions.
sampling 75% (156060939) splicing reads. 390374 splicing junctions. 199215 known splicing junctions. 191159 novel splicing junctions.
sampling 80% (166465002) splicing reads. 391974 splicing junctions. 199398 known splicing junctions. 192576 novel splicing junctions.
sampling 85% (176869065) splicing reads. 393261 splicing junctions. 199544 known splicing junctions. 193717 novel splicing junctions.
sampling 90% (187273127) splicing reads. 394222 splicing junctions. 199656 known splicing junctions. 194566 novel splicing junctions.
sampling 95% (197677190) splicing reads. 394874 splicing junctions. 199723 known splicing junctions. 195151 novel splicing junctions.
sampling 100% (208081253) splicing reads. 395185 splicing junctions. 199764 known splicing junctions. 195421 novel splicing junctions.