[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (12318718) splicing reads. 231148 splicing junctions. 175570 known splicing junctions. 55578 novel splicing junctions.
sampling 10% (24637437) splicing reads. 269009 splicing junctions. 185860 known splicing junctions. 83149 novel splicing junctions.
sampling 15% (36956155) splicing reads. 291736 splicing junctions. 190846 known splicing junctions. 100890 novel splicing junctions.
sampling 20% (49274874) splicing reads. 307310 splicing junctions. 193914 known splicing junctions. 113396 novel splicing junctions.
sampling 25% (61593592) splicing reads. 318733 splicing junctions. 195955 known splicing junctions. 122778 novel splicing junctions.
sampling 30% (73912311) splicing reads. 327441 splicing junctions. 197428 known splicing junctions. 130013 novel splicing junctions.
sampling 35% (86231029) splicing reads. 334377 splicing junctions. 198551 known splicing junctions. 135826 novel splicing junctions.
sampling 40% (98549748) splicing reads. 339971 splicing junctions. 199456 known splicing junctions. 140515 novel splicing junctions.
sampling 45% (110868466) splicing reads. 344474 splicing junctions. 200158 known splicing junctions. 144316 novel splicing junctions.
sampling 50% (123187185) splicing reads. 348207 splicing junctions. 200704 known splicing junctions. 147503 novel splicing junctions.
sampling 55% (135505903) splicing reads. 351316 splicing junctions. 201130 known splicing junctions. 150186 novel splicing junctions.
sampling 60% (147824622) splicing reads. 353918 splicing junctions. 201507 known splicing junctions. 152411 novel splicing junctions.
sampling 65% (160143340) splicing reads. 356126 splicing junctions. 201826 known splicing junctions. 154300 novel splicing junctions.
sampling 70% (172462059) splicing reads. 357939 splicing junctions. 202069 known splicing junctions. 155870 novel splicing junctions.
sampling 75% (184780777) splicing reads. 359419 splicing junctions. 202282 known splicing junctions. 157137 novel splicing junctions.
sampling 80% (197099496) splicing reads. 360583 splicing junctions. 202438 known splicing junctions. 158145 novel splicing junctions.
sampling 85% (209418214) splicing reads. 361552 splicing junctions. 202570 known splicing junctions. 158982 novel splicing junctions.
sampling 90% (221736933) splicing reads. 362325 splicing junctions. 202683 known splicing junctions. 159642 novel splicing junctions.
sampling 95% (234055651) splicing reads. 362881 splicing junctions. 202762 known splicing junctions. 160119 novel splicing junctions.
sampling 100% (246374370) splicing reads. 363192 splicing junctions. 202806 known splicing junctions. 160386 novel splicing junctions.