[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_bld_02_A23WKFTLT4_3.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (13112957) splicing reads. 240115 splicing junctions. 170406 known splicing junctions. 69709 novel splicing junctions.
sampling 10% (26225914) splicing reads. 286700 splicing junctions. 180603 known splicing junctions. 106097 novel splicing junctions.
sampling 15% (39338871) splicing reads. 315603 splicing junctions. 185761 known splicing junctions. 129842 novel splicing junctions.
sampling 20% (52451829) splicing reads. 335386 splicing junctions. 188729 known splicing junctions. 146657 novel splicing junctions.
sampling 25% (65564786) splicing reads. 350193 splicing junctions. 190798 known splicing junctions. 159395 novel splicing junctions.
sampling 30% (78677743) splicing reads. 361611 splicing junctions. 192281 known splicing junctions. 169330 novel splicing junctions.
sampling 35% (91790701) splicing reads. 370787 splicing junctions. 193400 known splicing junctions. 177387 novel splicing junctions.
sampling 40% (104903658) splicing reads. 377924 splicing junctions. 194202 known splicing junctions. 183722 novel splicing junctions.
sampling 45% (118016615) splicing reads. 383867 splicing junctions. 194909 known splicing junctions. 188958 novel splicing junctions.
sampling 50% (131129573) splicing reads. 388888 splicing junctions. 195468 known splicing junctions. 193420 novel splicing junctions.
sampling 55% (144242530) splicing reads. 392824 splicing junctions. 195916 known splicing junctions. 196908 novel splicing junctions.
sampling 60% (157355487) splicing reads. 396276 splicing junctions. 196288 known splicing junctions. 199988 novel splicing junctions.
sampling 65% (170468444) splicing reads. 399205 splicing junctions. 196591 known splicing junctions. 202614 novel splicing junctions.
sampling 70% (183581402) splicing reads. 401496 splicing junctions. 196832 known splicing junctions. 204664 novel splicing junctions.
sampling 75% (196694359) splicing reads. 403563 splicing junctions. 197062 known splicing junctions. 206501 novel splicing junctions.
sampling 80% (209807316) splicing reads. 405234 splicing junctions. 197221 known splicing junctions. 208013 novel splicing junctions.
sampling 85% (222920274) splicing reads. 406450 splicing junctions. 197328 known splicing junctions. 209122 novel splicing junctions.
sampling 90% (236033231) splicing reads. 407437 splicing junctions. 197426 known splicing junctions. 210011 novel splicing junctions.
sampling 95% (249146188) splicing reads. 408173 splicing junctions. 197518 known splicing junctions. 210655 novel splicing junctions.
sampling 100% (262259146) splicing reads. 408658 splicing junctions. 197567 known splicing junctions. 211091 novel splicing junctions.