[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_bst_01_02_A23WKFTLT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10395106) splicing reads. 231869 splicing junctions. 171523 known splicing junctions. 60346 novel splicing junctions.
sampling 10% (20790213) splicing reads. 273815 splicing junctions. 181886 known splicing junctions. 91929 novel splicing junctions.
sampling 15% (31185320) splicing reads. 299425 splicing junctions. 187011 known splicing junctions. 112414 novel splicing junctions.
sampling 20% (41580427) splicing reads. 317466 splicing junctions. 190132 known splicing junctions. 127334 novel splicing junctions.
sampling 25% (51975534) splicing reads. 330829 splicing junctions. 192270 known splicing junctions. 138559 novel splicing junctions.
sampling 30% (62370641) splicing reads. 340994 splicing junctions. 193811 known splicing junctions. 147183 novel splicing junctions.
sampling 35% (72765748) splicing reads. 349074 splicing junctions. 194916 known splicing junctions. 154158 novel splicing junctions.
sampling 40% (83160855) splicing reads. 355612 splicing junctions. 195748 known splicing junctions. 159864 novel splicing junctions.
sampling 45% (93555962) splicing reads. 360874 splicing junctions. 196465 known splicing junctions. 164409 novel splicing junctions.
sampling 50% (103951069) splicing reads. 365272 splicing junctions. 196993 known splicing junctions. 168279 novel splicing junctions.
sampling 55% (114346176) splicing reads. 369004 splicing junctions. 197467 known splicing junctions. 171537 novel splicing junctions.
sampling 60% (124741283) splicing reads. 372042 splicing junctions. 197866 known splicing junctions. 174176 novel splicing junctions.
sampling 65% (135136390) splicing reads. 374572 splicing junctions. 198174 known splicing junctions. 176398 novel splicing junctions.
sampling 70% (145531497) splicing reads. 376636 splicing junctions. 198415 known splicing junctions. 178221 novel splicing junctions.
sampling 75% (155926604) splicing reads. 378404 splicing junctions. 198603 known splicing junctions. 179801 novel splicing junctions.
sampling 80% (166321711) splicing reads. 379783 splicing junctions. 198774 known splicing junctions. 181009 novel splicing junctions.
sampling 85% (176716818) splicing reads. 380914 splicing junctions. 198891 known splicing junctions. 182023 novel splicing junctions.
sampling 90% (187111925) splicing reads. 381729 splicing junctions. 198988 known splicing junctions. 182741 novel splicing junctions.
sampling 95% (197507032) splicing reads. 382301 splicing junctions. 199059 known splicing junctions. 183242 novel splicing junctions.
sampling 100% (207902139) splicing reads. 382634 splicing junctions. 199102 known splicing junctions. 183532 novel splicing junctions.