[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_bld_02_A23WKFTLT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14954695) splicing reads. 258501 splicing junctions. 174882 known splicing junctions. 83619 novel splicing junctions.
sampling 10% (29909391) splicing reads. 318560 splicing junctions. 186408 known splicing junctions. 132152 novel splicing junctions.
sampling 15% (44864086) splicing reads. 359336 splicing junctions. 192336 known splicing junctions. 167000 novel splicing junctions.
sampling 20% (59818782) splicing reads. 389770 splicing junctions. 196119 known splicing junctions. 193651 novel splicing junctions.
sampling 25% (74773478) splicing reads. 413500 splicing junctions. 198757 known splicing junctions. 214743 novel splicing junctions.
sampling 30% (89728173) splicing reads. 432384 splicing junctions. 200695 known splicing junctions. 231689 novel splicing junctions.
sampling 35% (104682869) splicing reads. 448062 splicing junctions. 202220 known splicing junctions. 245842 novel splicing junctions.
sampling 40% (119637565) splicing reads. 460976 splicing junctions. 203366 known splicing junctions. 257610 novel splicing junctions.
sampling 45% (134592260) splicing reads. 471789 splicing junctions. 204279 known splicing junctions. 267510 novel splicing junctions.
sampling 50% (149546956) splicing reads. 481007 splicing junctions. 205048 known splicing junctions. 275959 novel splicing junctions.
sampling 55% (164501652) splicing reads. 488718 splicing junctions. 205674 known splicing junctions. 283044 novel splicing junctions.
sampling 60% (179456347) splicing reads. 495430 splicing junctions. 206187 known splicing junctions. 289243 novel splicing junctions.
sampling 65% (194411043) splicing reads. 500983 splicing junctions. 206625 known splicing junctions. 294358 novel splicing junctions.
sampling 70% (209365739) splicing reads. 505628 splicing junctions. 207004 known splicing junctions. 298624 novel splicing junctions.
sampling 75% (224320434) splicing reads. 509632 splicing junctions. 207322 known splicing junctions. 302310 novel splicing junctions.
sampling 80% (239275130) splicing reads. 512973 splicing junctions. 207573 known splicing junctions. 305400 novel splicing junctions.
sampling 85% (254229826) splicing reads. 515662 splicing junctions. 207777 known splicing junctions. 307885 novel splicing junctions.
sampling 90% (269184521) splicing reads. 517665 splicing junctions. 207909 known splicing junctions. 309756 novel splicing junctions.
sampling 95% (284139217) splicing reads. 519277 splicing junctions. 208016 known splicing junctions. 311261 novel splicing junctions.
sampling 100% (299093913) splicing reads. 520310 splicing junctions. 208091 known splicing junctions. 312219 novel splicing junctions.