+ mkdir BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2 + all_qc -bam BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai 0it [00:00, ?it/s] 127682it [00:00, 1276702.51it/s] 283624it [00:00, 1442950.83it/s] 448875it [00:00, 1538584.93it/s] 617407it [00:00, 1596473.28it/s] 788971it [00:00, 1639413.35it/s] 962388it [00:00, 1671600.09it/s] 1136801it [00:00, 1695273.11it/s] 1310362it [00:00, 1708082.02it/s] 1485963it [00:00, 1723005.85it/s] 1661364it [00:01, 1732546.87it/s] 1836880it [00:01, 1739436.10it/s] 2010824it [00:01, 1736409.05it/s] 2187539it [00:01, 1745680.24it/s] 2362109it [00:01, 1695573.85it/s] 2536576it [00:01, 1710037.38it/s] 2716286it [00:01, 1735836.03it/s] 2893120it [00:01, 1745478.70it/s] 3067826it [00:01, 1579580.02it/s] 3228833it [00:01, 1492131.22it/s] 3391967it [00:02, 1529951.96it/s] 3555964it [00:02, 1560706.63it/s] 3722698it [00:02, 1591132.76it/s] 3883248it [00:02, 1573759.87it/s] 4041797it [00:02, 1577129.66it/s] 4200223it [00:02, 1563471.22it/s] 4358196it [00:02, 1568109.01it/s] 4515367it [00:02, 1554283.65it/s] 4671055it [00:02, 1553467.17it/s] 4831145it [00:02, 1567455.63it/s] 4988037it [00:03, 1451761.74it/s] 5134945it [00:03, 1370253.93it/s] 5273803it [00:03, 1319854.39it/s] 5407140it [00:03, 1284841.69it/s] 5536510it [00:03, 1263767.36it/s] 5663422it [00:03, 1246407.13it/s] 5788373it [00:03, 1235387.40it/s] 5927734it [00:03, 1280425.73it/s][W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai 6078487it [00:03, 1345999.58it/s] 6222262it [00:04, 1372790.71it/s] 6359957it [00:04, 1372904.59it/s] 6504715it [00:04, 1394981.41it/s] 6657196it [00:04, 1433535.02it/s] 6800749it [00:04, 1423536.19it/s] 6948972it [00:04, 1440943.40it/s] 7094166it [00:04, 1444187.32it/s] 7241929it [00:04, 1454099.64it/s] 7390784it [00:04, 1464361.20it/s] 7542853it [00:04, 1481041.93it/s] 7690996it [00:05, 1479625.68it/s] 7844807it [00:05, 1497069.56it/s] 7994537it [00:05, 1497068.45it/s] 8144260it [00:05, 1485504.16it/s] 8292841it [00:05, 1460430.93it/s] 8441534it [00:05, 1468115.88it/s][W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai 8593246it [00:05, 1482610.24it/s] 8741583it [00:05, 1469153.93it/s] 8889085it [00:05, 1470864.78it/s] 9037493it [00:05, 1474762.49it/s] 9185296it [00:06, 1475701.61it/s][W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai 9333871it [00:06, 1478556.39it/s] 9485615it [00:06, 1490145.11it/s] 9637477it [00:06, 1498629.06it/s] 9787355it [00:06, 1484157.76it/s] 9946705it [00:06, 1516646.00it/s] 10098424it [00:06, 1515208.26it/s] 10249982it [00:06, 1493595.15it/s] 10399429it [00:06, 1464778.05it/s] 10546058it [00:07, 1457970.39it/s] 10691953it [00:07, 1441526.84it/s] 10836187it [00:07, 1432863.64it/s] 10979524it [00:07, 1417758.22it/s] 11121343it [00:07, 1414503.79it/s] 11262819it [00:07, 1411878.66it/s] 11406245it [00:07, 1418471.92it/s] 11548153it [00:07, 1418604.82it/s] 11690352it [00:07, 1419511.32it/s] 11835842it [00:07, 1430050.14it/s] 11985520it [00:08, 1449850.89it/s] 12130520it [00:08, 1449163.79it/s] 12280151it [00:08, 1463245.47it/s] 12426486it [00:08, 1434897.46it/s] 12570102it [00:08, 1386272.12it/s] 12713509it [00:08, 1400100.86it/s] 12853840it [00:08, 1400818.71it/s] 13005197it [00:08, 1434037.95it/s] 13152828it [00:08, 1446466.04it/s] 13300181it [00:08, 1454489.27it/s] 13445754it [00:09, 1437510.31it/s] 13589628it [00:09, 1426796.50it/s] 13736446it [00:09, 1439012.09it/s][W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai 13887911it [00:09, 1461434.38it/s] 14036262it [00:09, 1467987.88it/s] 14186494it [00:09, 1478208.51it/s][W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai 14334363it [00:09, 1475314.95it/s] 14481929it [00:09, 1420209.72it/s] 14628780it [00:09, 1434251.67it/s] 14713457it [00:09, 1484602.91it/s] [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md.bai + merge_qc -folders BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2/BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.md -output BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2/BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2_full_basic_stats.tsv + head -n1 BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2/BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2/BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2_full_basic_stats.tsv