[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_brn_01_A23WKFTLT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (9508623) splicing reads. 209222 splicing junctions. 167492 known splicing junctions. 41730 novel splicing junctions.
sampling 10% (19017247) splicing reads. 235265 splicing junctions. 176314 known splicing junctions. 58951 novel splicing junctions.
sampling 15% (28525871) splicing reads. 249495 splicing junctions. 180232 known splicing junctions. 69263 novel splicing junctions.
sampling 20% (38034495) splicing reads. 258461 splicing junctions. 182454 known splicing junctions. 76007 novel splicing junctions.
sampling 25% (47543119) splicing reads. 264737 splicing junctions. 183954 known splicing junctions. 80783 novel splicing junctions.
sampling 30% (57051743) splicing reads. 269468 splicing junctions. 185067 known splicing junctions. 84401 novel splicing junctions.
sampling 35% (66560367) splicing reads. 273040 splicing junctions. 185847 known splicing junctions. 87193 novel splicing junctions.
sampling 40% (76068991) splicing reads. 275724 splicing junctions. 186413 known splicing junctions. 89311 novel splicing junctions.
sampling 45% (85577615) splicing reads. 277921 splicing junctions. 186842 known splicing junctions. 91079 novel splicing junctions.
sampling 50% (95086239) splicing reads. 279704 splicing junctions. 187165 known splicing junctions. 92539 novel splicing junctions.
sampling 55% (104594863) splicing reads. 281289 splicing junctions. 187469 known splicing junctions. 93820 novel splicing junctions.
sampling 60% (114103487) splicing reads. 282545 splicing junctions. 187679 known splicing junctions. 94866 novel splicing junctions.
sampling 65% (123612111) splicing reads. 283647 splicing junctions. 187878 known splicing junctions. 95769 novel splicing junctions.
sampling 70% (133120735) splicing reads. 284499 splicing junctions. 188014 known splicing junctions. 96485 novel splicing junctions.
sampling 75% (142629359) splicing reads. 285297 splicing junctions. 188153 known splicing junctions. 97144 novel splicing junctions.
sampling 80% (152137983) splicing reads. 285877 splicing junctions. 188273 known splicing junctions. 97604 novel splicing junctions.
sampling 85% (161646607) splicing reads. 286401 splicing junctions. 188357 known splicing junctions. 98044 novel splicing junctions.
sampling 90% (171155231) splicing reads. 286788 splicing junctions. 188431 known splicing junctions. 98357 novel splicing junctions.
sampling 95% (180663855) splicing reads. 287042 splicing junctions. 188480 known splicing junctions. 98562 novel splicing junctions.
sampling 100% (190172479) splicing reads. 287198 splicing junctions. 188514 known splicing junctions. 98684 novel splicing junctions.