[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_bst_02_A23WKFTLT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (17004637) splicing reads. 255346 splicing junctions. 181880 known splicing junctions. 73466 novel splicing junctions.
sampling 10% (34009275) splicing reads. 309502 splicing junctions. 193934 known splicing junctions. 115568 novel splicing junctions.
sampling 15% (51013913) splicing reads. 346343 splicing junctions. 200238 known splicing junctions. 146105 novel splicing junctions.
sampling 20% (68018551) splicing reads. 373934 splicing junctions. 204285 known splicing junctions. 169649 novel splicing junctions.
sampling 25% (85023189) splicing reads. 395556 splicing junctions. 207057 known splicing junctions. 188499 novel splicing junctions.
sampling 30% (102027827) splicing reads. 413006 splicing junctions. 209192 known splicing junctions. 203814 novel splicing junctions.
sampling 35% (119032464) splicing reads. 427481 splicing junctions. 210744 known splicing junctions. 216737 novel splicing junctions.
sampling 40% (136037102) splicing reads. 439437 splicing junctions. 212085 known splicing junctions. 227352 novel splicing junctions.
sampling 45% (153041740) splicing reads. 449714 splicing junctions. 213167 known splicing junctions. 236547 novel splicing junctions.
sampling 50% (170046378) splicing reads. 458500 splicing junctions. 214075 known splicing junctions. 244425 novel splicing junctions.
sampling 55% (187051016) splicing reads. 465903 splicing junctions. 214789 known splicing junctions. 251114 novel splicing junctions.
sampling 60% (204055654) splicing reads. 472341 splicing junctions. 215465 known splicing junctions. 256876 novel splicing junctions.
sampling 65% (221060292) splicing reads. 477707 splicing junctions. 215976 known splicing junctions. 261731 novel splicing junctions.
sampling 70% (238064929) splicing reads. 482388 splicing junctions. 216437 known splicing junctions. 265951 novel splicing junctions.
sampling 75% (255069567) splicing reads. 486279 splicing junctions. 216791 known splicing junctions. 269488 novel splicing junctions.
sampling 80% (272074205) splicing reads. 489501 splicing junctions. 217074 known splicing junctions. 272427 novel splicing junctions.
sampling 85% (289078843) splicing reads. 492188 splicing junctions. 217297 known splicing junctions. 274891 novel splicing junctions.
sampling 90% (306083481) splicing reads. 494308 splicing junctions. 217474 known splicing junctions. 276834 novel splicing junctions.
sampling 95% (323088119) splicing reads. 495959 splicing junctions. 217631 known splicing junctions. 278328 novel splicing junctions.
sampling 100% (340092757) splicing reads. 497118 splicing junctions. 217734 known splicing junctions. 279384 novel splicing junctions.