[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_bst_01_01_A23WKFTLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (13242034) splicing reads. 249925 splicing junctions. 176889 known splicing junctions. 73036 novel splicing junctions.
sampling 10% (26484068) splicing reads. 302884 splicing junctions. 187973 known splicing junctions. 114911 novel splicing junctions.
sampling 15% (39726103) splicing reads. 338634 splicing junctions. 193724 known splicing junctions. 144910 novel splicing junctions.
sampling 20% (52968137) splicing reads. 365197 splicing junctions. 197449 known splicing junctions. 167748 novel splicing junctions.
sampling 25% (66210171) splicing reads. 385466 splicing junctions. 199984 known splicing junctions. 185482 novel splicing junctions.
sampling 30% (79452206) splicing reads. 401974 splicing junctions. 201887 known splicing junctions. 200087 novel splicing junctions.
sampling 35% (92694240) splicing reads. 415138 splicing junctions. 203326 known splicing junctions. 211812 novel splicing junctions.
sampling 40% (105936274) splicing reads. 426220 splicing junctions. 204433 known splicing junctions. 221787 novel splicing junctions.
sampling 45% (119178309) splicing reads. 435409 splicing junctions. 205314 known splicing junctions. 230095 novel splicing junctions.
sampling 50% (132420343) splicing reads. 443201 splicing junctions. 206051 known splicing junctions. 237150 novel splicing junctions.
sampling 55% (145662377) splicing reads. 449834 splicing junctions. 206699 known splicing junctions. 243135 novel splicing junctions.
sampling 60% (158904412) splicing reads. 455330 splicing junctions. 207257 known splicing junctions. 248073 novel splicing junctions.
sampling 65% (172146446) splicing reads. 459936 splicing junctions. 207657 known splicing junctions. 252279 novel splicing junctions.
sampling 70% (185388480) splicing reads. 463882 splicing junctions. 208033 known splicing junctions. 255849 novel splicing junctions.
sampling 75% (198630515) splicing reads. 467202 splicing junctions. 208316 known splicing junctions. 258886 novel splicing junctions.
sampling 80% (211872549) splicing reads. 469856 splicing junctions. 208579 known splicing junctions. 261277 novel splicing junctions.
sampling 85% (225114583) splicing reads. 471909 splicing junctions. 208749 known splicing junctions. 263160 novel splicing junctions.
sampling 90% (238356618) splicing reads. 473558 splicing junctions. 208909 known splicing junctions. 264649 novel splicing junctions.
sampling 95% (251598652) splicing reads. 474693 splicing junctions. 209006 known splicing junctions. 265687 novel splicing junctions.
sampling 100% (264840687) splicing reads. 475356 splicing junctions. 209066 known splicing junctions. 266290 novel splicing junctions.