[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_bst_01_03_A23WKFTLT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (12852482) splicing reads. 248811 splicing junctions. 176297 known splicing junctions. 72514 novel splicing junctions.
sampling 10% (25704964) splicing reads. 295471 splicing junctions. 186331 known splicing junctions. 109140 novel splicing junctions.
sampling 15% (38557447) splicing reads. 324649 splicing junctions. 191302 known splicing junctions. 133347 novel splicing junctions.
sampling 20% (51409929) splicing reads. 344824 splicing junctions. 194303 known splicing junctions. 150521 novel splicing junctions.
sampling 25% (64262412) splicing reads. 359764 splicing junctions. 196422 known splicing junctions. 163342 novel splicing junctions.
sampling 30% (77114894) splicing reads. 370984 splicing junctions. 197940 known splicing junctions. 173044 novel splicing junctions.
sampling 35% (89967376) splicing reads. 379962 splicing junctions. 199022 known splicing junctions. 180940 novel splicing junctions.
sampling 40% (102819859) splicing reads. 387417 splicing junctions. 199884 known splicing junctions. 187533 novel splicing junctions.
sampling 45% (115672341) splicing reads. 393347 splicing junctions. 200544 known splicing junctions. 192803 novel splicing junctions.
sampling 50% (128524824) splicing reads. 398103 splicing junctions. 201058 known splicing junctions. 197045 novel splicing junctions.
sampling 55% (141377306) splicing reads. 402241 splicing junctions. 201521 known splicing junctions. 200720 novel splicing junctions.
sampling 60% (154229788) splicing reads. 405534 splicing junctions. 201870 known splicing junctions. 203664 novel splicing junctions.
sampling 65% (167082271) splicing reads. 408407 splicing junctions. 202149 known splicing junctions. 206258 novel splicing junctions.
sampling 70% (179934753) splicing reads. 410774 splicing junctions. 202398 known splicing junctions. 208376 novel splicing junctions.
sampling 75% (192787236) splicing reads. 412697 splicing junctions. 202602 known splicing junctions. 210095 novel splicing junctions.
sampling 80% (205639718) splicing reads. 414253 splicing junctions. 202769 known splicing junctions. 211484 novel splicing junctions.
sampling 85% (218492200) splicing reads. 415492 splicing junctions. 202917 known splicing junctions. 212575 novel splicing junctions.
sampling 90% (231344683) splicing reads. 416441 splicing junctions. 203028 known splicing junctions. 213413 novel splicing junctions.
sampling 95% (244197165) splicing reads. 417128 splicing junctions. 203097 known splicing junctions. 214031 novel splicing junctions.
sampling 100% (257049648) splicing reads. 417493 splicing junctions. 203133 known splicing junctions. 214360 novel splicing junctions.