[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_bst_01_01_A23WKFTLT4_3.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14094385) splicing reads. 237479 splicing junctions. 172460 known splicing junctions. 65019 novel splicing junctions.
sampling 10% (28188771) splicing reads. 270703 splicing junctions. 180428 known splicing junctions. 90275 novel splicing junctions.
sampling 15% (42283157) splicing reads. 288256 splicing junctions. 183888 known splicing junctions. 104368 novel splicing junctions.
sampling 20% (56377542) splicing reads. 299231 splicing junctions. 185779 known splicing junctions. 113452 novel splicing junctions.
sampling 25% (70471928) splicing reads. 306553 splicing junctions. 186948 known splicing junctions. 119605 novel splicing junctions.
sampling 30% (84566314) splicing reads. 311986 splicing junctions. 187817 known splicing junctions. 124169 novel splicing junctions.
sampling 35% (98660699) splicing reads. 316112 splicing junctions. 188419 known splicing junctions. 127693 novel splicing junctions.
sampling 40% (112755085) splicing reads. 319412 splicing junctions. 188920 known splicing junctions. 130492 novel splicing junctions.
sampling 45% (126849471) splicing reads. 322067 splicing junctions. 189249 known splicing junctions. 132818 novel splicing junctions.
sampling 50% (140943857) splicing reads. 324162 splicing junctions. 189535 known splicing junctions. 134627 novel splicing junctions.
sampling 55% (155038242) splicing reads. 326036 splicing junctions. 189785 known splicing junctions. 136251 novel splicing junctions.
sampling 60% (169132628) splicing reads. 327519 splicing junctions. 189987 known splicing junctions. 137532 novel splicing junctions.
sampling 65% (183227014) splicing reads. 328804 splicing junctions. 190151 known splicing junctions. 138653 novel splicing junctions.
sampling 70% (197321399) splicing reads. 329845 splicing junctions. 190272 known splicing junctions. 139573 novel splicing junctions.
sampling 75% (211415785) splicing reads. 330714 splicing junctions. 190377 known splicing junctions. 140337 novel splicing junctions.
sampling 80% (225510171) splicing reads. 331429 splicing junctions. 190468 known splicing junctions. 140961 novel splicing junctions.
sampling 85% (239604556) splicing reads. 331985 splicing junctions. 190527 known splicing junctions. 141458 novel splicing junctions.
sampling 90% (253698942) splicing reads. 332422 splicing junctions. 190573 known splicing junctions. 141849 novel splicing junctions.
sampling 95% (267793328) splicing reads. 332709 splicing junctions. 190608 known splicing junctions. 142101 novel splicing junctions.
sampling 100% (281887714) splicing reads. 332866 splicing junctions. 190624 known splicing junctions. 142242 novel splicing junctions.