STAR --genomeDir ref_genome.fa.star.idx --readFilesIn input1/BVT_FFPE_TRNA_bld_01_A23WKFTLT4_2_1.fastp.fastq.gz input2/BVT_FFPE_TRNA_bld_01_A23WKFTLT4_2_2.fastp.fastq.gz --runThreadN 24 --outFileNamePrefix BVT_FFPE_TRNA_bld_01_A23WKFTLT4_2. --sjdbGTFfile ref_annot.gtf --outSAMattrRGline ID:BVT_FFPE_TRNA_bld_01_A23WKFTLT4_2 SM:BVT_FFPE_TRNA_bld_01_A23WKFTLT4_2 --twopassMode None --chimSegmentMin 12 --chimJunctionOverhangMin 5 --chimMultimapNmax 50 --chimScoreJunctionNonGTAG -4 --chimScoreSeparation 1 --alignIntronMax 140000 --outReadsUnmapped None --readFilesCommand zcat --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --outSAMunmapped Within --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --chimMultimapScoreRange 3 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLmin 30 --alignSplicedMateMapLminOverLmate 0.5 --quantMode GeneCounts --runRNGseed 1 --chimOutType Junctions WithinBAM --limitBAMsortRAM 38654705664
STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source
Jun 08 07:26:58 ..... started STAR run
Jun 08 07:26:58 ..... loading genome
Jun 08 07:27:15 ..... processing annotations GTF
Jun 08 07:27:25 ..... inserting junctions into the genome indices
Jun 08 07:28:33 ..... started mapping
Jun 08 07:42:13 ..... finished mapping
Jun 08 07:42:18 ..... started sorting BAM
Jun 08 07:43:25 ..... finished successfully