[W::hts_idx_load3] The index file is older than the data file: BVT_FFPE_TRNA_bld_02_A23WKFTLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14335303) splicing reads. 250751 splicing junctions. 174010 known splicing junctions. 76741 novel splicing junctions.
sampling 10% (28670606) splicing reads. 308519 splicing junctions. 185991 known splicing junctions. 122528 novel splicing junctions.
sampling 15% (43005909) splicing reads. 348993 splicing junctions. 192212 known splicing junctions. 156781 novel splicing junctions.
sampling 20% (57341212) splicing reads. 379446 splicing junctions. 196203 known splicing junctions. 183243 novel splicing junctions.
sampling 25% (71676515) splicing reads. 403725 splicing junctions. 199149 known splicing junctions. 204576 novel splicing junctions.
sampling 30% (86011818) splicing reads. 423760 splicing junctions. 201263 known splicing junctions. 222497 novel splicing junctions.
sampling 35% (100347121) splicing reads. 440340 splicing junctions. 202941 known splicing junctions. 237399 novel splicing junctions.
sampling 40% (114682424) splicing reads. 454262 splicing junctions. 204254 known splicing junctions. 250008 novel splicing junctions.
sampling 45% (129017727) splicing reads. 465998 splicing junctions. 205316 known splicing junctions. 260682 novel splicing junctions.
sampling 50% (143353030) splicing reads. 476184 splicing junctions. 206199 known splicing junctions. 269985 novel splicing junctions.
sampling 55% (157688333) splicing reads. 484786 splicing junctions. 206948 known splicing junctions. 277838 novel splicing junctions.
sampling 60% (172023636) splicing reads. 492188 splicing junctions. 207589 known splicing junctions. 284599 novel splicing junctions.
sampling 65% (186358939) splicing reads. 498505 splicing junctions. 208080 known splicing junctions. 290425 novel splicing junctions.
sampling 70% (200694242) splicing reads. 503807 splicing junctions. 208491 known splicing junctions. 295316 novel splicing junctions.
sampling 75% (215029545) splicing reads. 508469 splicing junctions. 208855 known splicing junctions. 299614 novel splicing junctions.
sampling 80% (229364848) splicing reads. 512296 splicing junctions. 209144 known splicing junctions. 303152 novel splicing junctions.
sampling 85% (243700151) splicing reads. 515421 splicing junctions. 209377 known splicing junctions. 306044 novel splicing junctions.
sampling 90% (258035454) splicing reads. 517767 splicing junctions. 209557 known splicing junctions. 308210 novel splicing junctions.
sampling 95% (272370757) splicing reads. 519588 splicing junctions. 209710 known splicing junctions. 309878 novel splicing junctions.
sampling 100% (286706060) splicing reads. 520736 splicing junctions. 209783 known splicing junctions. 310953 novel splicing junctions.