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BCL CONVERT QC REPORT - DETAILED
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1. % UNDETERMINED PER LANE
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%_Undetermined Undetermined_Reads Total_Reads
Lane
1 6.62 257820405 3894261515
2 6.65 259496248 3899701796
3 6.73 263462293 3917603373
4 7.11 277827173 3905745308
5 6.86 269126962 3923505923
6 6.51 256291739 3934679671
7 6.32 251780692 3981720921
8 6.57 260393918 3961600312
2. SAMPLES WITH LOW READS (NON-NTC)
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No samples found with low reads
3. NTC SAMPLES WITH HIGH READS
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No NTC samples found with high reads
4. TOP UNKNOWN BARCODES
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Found 117 significant unknown barcodes:
Lane index index2 # Reads % of Unknown Barcodes % of All Reads Percentage_of_Lane Percentage_of_Total
0 1 AGCCTCTGCT GGGGGGGGGG 8126639 0.031521 0.002087 0.208682 0.025866
1 1 GCATTCCTTC GGGGGGGGGG 5307065 0.020584 0.001363 0.136279 0.016891
2 1 CCAACTGTAT GGGGGGGGGG 5231376 0.020291 0.001343 0.134336 0.016650
3 1 AGACGACAGA GGGGGGGGGG 4333710 0.016809 0.001113 0.111285 0.013793
4 1 GTGACGCACA GGGGGGGGGG 3920802 0.015207 0.001007 0.100682 0.012479
5 1 ACCAGGAATT GGGGGGGGGG 3087441 0.011975 0.000793 0.079282 0.009827
6 1 AAGGTGTACT GGGGGGGGGG 3038941 0.011787 0.000780 0.078036 0.009672
7 1 TGTGATCGCG GGGGGGGGGG 2867302 0.011121 0.000736 0.073629 0.009126
8 1 ACTCGTCCAG GGGGGGGGGG 2798198 0.010853 0.000719 0.071854 0.008906
9 1 CGGATCATTA GGGGGGGGGG 2785855 0.010805 0.000715 0.071537 0.008867
10 1 AACGCTCTGA GGGGGGGGGG 2423346 0.009399 0.000622 0.062229 0.007713
11 1 GATAGGTGAA GGGGGGGGGG 2368340 0.009186 0.000608 0.060816 0.007538
12 1 GAGTAGTGAC GGGGGGGGGG 2160731 0.008381 0.000555 0.055485 0.006877
13 1 GCAGAAGTCT GGGGGGGGGG 2155817 0.008362 0.000554 0.055359 0.006862
14 1 TCACCGTGGT GGGGGGGGGG 2108173 0.008177 0.000541 0.054135 0.006710
15 2 AGCCTCTGCT GGGGGGGGGG 7988080 0.030783 0.002048 0.204838 0.025425
16 2 CCAACTGTAT GGGGGGGGGG 5151528 0.019852 0.001321 0.132101 0.016396
17 2 GCATTCCTTC GGGGGGGGGG 5115608 0.019714 0.001312 0.131179 0.016282
18 2 AGACGACAGA GGGGGGGGGG 4241168 0.016344 0.001088 0.108756 0.013499
19 2 GTGACGCACA GGGGGGGGGG 3823093 0.014733 0.000980 0.098036 0.012168
20 2 ACCAGGAATT GGGGGGGGGG 3265023 0.012582 0.000837 0.083725 0.010392
21 2 AAGGTGTACT GGGGGGGGGG 3127400 0.012052 0.000802 0.080196 0.009954
22 2 ACTCGTCCAG GGGGGGGGGG 3022519 0.011648 0.000775 0.077506 0.009620
23 2 TGTGATCGCG GGGGGGGGGG 2735708 0.010542 0.000702 0.070152 0.008707
24 2 CGGATCATTA GGGGGGGGGG 2663871 0.010266 0.000683 0.068310 0.008479
25 2 AACGCTCTGA GGGGGGGGGG 2398926 0.009245 0.000615 0.061516 0.007635
26 2 GAGTAGTGAC GGGGGGGGGG 2308700 0.008897 0.000592 0.059202 0.007348
27 2 GATAGGTGAA GGGGGGGGGG 2292882 0.008836 0.000588 0.058796 0.007298
28 2 TCACCGTGGT GGGGGGGGGG 2052717 0.007910 0.000526 0.052638 0.006533
29 2 GCAGAAGTCT GGGGGGGGGG 2052335 0.007909 0.000526 0.052628 0.006532
30 3 AGCCTCTGCT GGGGGGGGGG 7632942 0.028972 0.001948 0.194837 0.024294
31 3 CCAACTGTAT GGGGGGGGGG 5001830 0.018985 0.001277 0.127676 0.015920
32 3 GCATTCCTTC GGGGGGGGGG 4831729 0.018339 0.001233 0.123334 0.015378
33 3 AGACGACAGA GGGGGGGGGG 4049238 0.015369 0.001034 0.103360 0.012888
34 3 GTGACGCACA GGGGGGGGGG 3798816 0.014419 0.000970 0.096968 0.012091
35 3 ACCAGGAATT GGGGGGGGGG 3281711 0.012456 0.000838 0.083768 0.010445
36 3 AAGGTGTACT GGGGGGGGGG 3167125 0.012021 0.000808 0.080843 0.010080
37 3 ACTCGTCCAG GGGGGGGGGG 3048513 0.011571 0.000778 0.077816 0.009703
38 3 CGGATCATTA GGGGGGGGGG 2668730 0.010129 0.000681 0.068121 0.008494
39 3 TGTGATCGCG GGGGGGGGGG 2623294 0.009957 0.000670 0.066962 0.008349
40 3 AACGCTCTGA GGGGGGGGGG 2453184 0.009311 0.000626 0.062620 0.007808
41 3 GAGTAGTGAC GGGGGGGGGG 2282793 0.008665 0.000583 0.058270 0.007266
42 3 GATAGGTGAA GGGGGGGGGG 2187708 0.008304 0.000558 0.055843 0.006963
43 3 GCAGAAGTCT GGGGGGGGGG 2034452 0.007722 0.000519 0.051931 0.006475
44 3 TCACCGTGGT GGGGGGGGGG 2003638 0.007605 0.000511 0.051144 0.006377
45 4 AGCCTCTGCT GGGGGGGGGG 7465598 0.026871 0.001911 0.191144 0.023762
46 4 CCAACTGTAT GGGGGGGGGG 4913008 0.017684 0.001258 0.125789 0.015637
47 4 GCATTCCTTC GGGGGGGGGG 4602704 0.016567 0.001178 0.117844 0.014650
48 4 AGACGACAGA GGGGGGGGGG 3969967 0.014289 0.001016 0.101644 0.012636
49 4 GTGACGCACA GGGGGGGGGG 3766880 0.013558 0.000964 0.096445 0.011989
50 4 ACCAGGAATT GGGGGGGGGG 3370480 0.012132 0.000863 0.086295 0.010728
51 4 AAGGTGTACT GGGGGGGGGG 3338659 0.012017 0.000855 0.085481 0.010626
52 4 ACTCGTCCAG GGGGGGGGGG 3135281 0.011285 0.000803 0.080274 0.009979
53 4 CGGATCATTA GGGGGGGGGG 2765104 0.009953 0.000708 0.070796 0.008801
54 4 TGTGATCGCG GGGGGGGGGG 2746087 0.009884 0.000703 0.070309 0.008740
55 4 AACGCTCTGA GGGGGGGGGG 2548718 0.009174 0.000653 0.065256 0.008112
56 4 GAGTAGTGAC GGGGGGGGGG 2299249 0.008276 0.000589 0.058868 0.007318
57 4 GCAGAAGTCT GGGGGGGGGG 2148606 0.007734 0.000550 0.055011 0.006839
58 4 GCACTACCAG GGGGGGGGGG 2143384 0.007715 0.000549 0.054878 0.006822
59 4 GATAGGTGAA GGGGGGGGGG 2124834 0.007648 0.000544 0.054403 0.006763
60 4 TCTCCGTACT GGGGGGGGGG 2022059 0.007278 0.000518 0.051771 0.006436
61 5 AGCCTCTGCT GGGGGGGGGG 7373040 0.027396 0.001879 0.187920 0.023467
62 5 CCAACTGTAT GGGGGGGGGG 4864630 0.018076 0.001240 0.123987 0.015483
63 5 GCATTCCTTC GGGGGGGGGG 4653505 0.017291 0.001186 0.118606 0.014811
64 5 AGACGACAGA GGGGGGGGGG 3897363 0.014482 0.000993 0.099334 0.012405
65 5 GTGACGCACA GGGGGGGGGG 3697185 0.013738 0.000942 0.094232 0.011767
66 5 ACCAGGAATT GGGGGGGGGG 3270331 0.012152 0.000834 0.083352 0.010409
67 5 AAGGTGTACT GGGGGGGGGG 3198407 0.011884 0.000815 0.081519 0.010180
68 5 ACTCGTCCAG GGGGGGGGGG 3018105 0.011214 0.000769 0.076924 0.009606
69 5 CGGATCATTA GGGGGGGGGG 2676989 0.009947 0.000682 0.068230 0.008520
70 5 TGTGATCGCG GGGGGGGGGG 2622743 0.009745 0.000668 0.066847 0.008348
71 5 AACGCTCTGA GGGGGGGGGG 2461767 0.009147 0.000627 0.062744 0.007835
72 5 GAGTAGTGAC GGGGGGGGGG 2247226 0.008350 0.000573 0.057276 0.007152
73 5 GATAGGTGAA GGGGGGGGGG 2118861 0.007873 0.000540 0.054004 0.006744
74 5 GCAGAAGTCT GGGGGGGGGG 2038861 0.007576 0.000520 0.051965 0.006489
75 6 AGCCTCTGCT GGGGGGGGGG 7628004 0.029763 0.001939 0.193866 0.024278
76 6 CCAACTGTAT GGGGGGGGGG 5013015 0.019560 0.001274 0.127406 0.015955
77 6 GCATTCCTTC GGGGGGGGGG 4901161 0.019123 0.001246 0.124563 0.015599
78 6 AGACGACAGA GGGGGGGGGG 4061502 0.015847 0.001032 0.103223 0.012927
79 6 GTGACGCACA GGGGGGGGGG 3715019 0.014495 0.000944 0.094417 0.011824
80 6 ACCAGGAATT GGGGGGGGGG 3178789 0.012403 0.000808 0.080789 0.010117
81 6 AAGGTGTACT GGGGGGGGGG 3060936 0.011943 0.000778 0.077794 0.009742
82 6 ACTCGTCCAG GGGGGGGGGG 2902790 0.011326 0.000738 0.073774 0.009239
83 6 CGGATCATTA GGGGGGGGGG 2587411 0.010096 0.000658 0.065759 0.008235
84 6 TGTGATCGCG GGGGGGGGGG 2571229 0.010032 0.000653 0.065348 0.008184
85 6 AACGCTCTGA GGGGGGGGGG 2316655 0.009039 0.000589 0.058878 0.007373
86 6 GAGTAGTGAC GGGGGGGGGG 2243652 0.008754 0.000570 0.057022 0.007141
87 6 GATAGGTGAA GGGGGGGGGG 2221639 0.008668 0.000565 0.056463 0.007071
88 6 TCACCGTGGT GGGGGGGGGG 1980556 0.007728 0.000503 0.050336 0.006304
89 7 AGCCTCTGCT GGGGGGGGGG 7799811 0.030979 0.001959 0.195890 0.024825
90 7 CCAACTGTAT GGGGGGGGGG 5090268 0.020217 0.001278 0.127841 0.016201
91 7 GCATTCCTTC GGGGGGGGGG 4970329 0.019741 0.001248 0.124829 0.015820
92 7 AGACGACAGA GGGGGGGGGG 4199572 0.016679 0.001055 0.105471 0.013366
93 7 GTGACGCACA GGGGGGGGGG 3705573 0.014717 0.000931 0.093065 0.011794
94 7 ACCAGGAATT GGGGGGGGGG 3118911 0.012387 0.000783 0.078331 0.009927
95 7 AAGGTGTACT GGGGGGGGGG 3006231 0.011940 0.000755 0.075501 0.009568
96 7 ACTCGTCCAG GGGGGGGGGG 2853468 0.011333 0.000717 0.071664 0.009082
97 7 TGTGATCGCG GGGGGGGGGG 2569736 0.010206 0.000645 0.064538 0.008179
98 7 CGGATCATTA GGGGGGGGGG 2540881 0.010092 0.000638 0.063814 0.008087
99 7 AACGCTCTGA GGGGGGGGGG 2256384 0.008962 0.000567 0.056669 0.007182
100 7 GATAGGTGAA GGGGGGGGGG 2228002 0.008849 0.000560 0.055956 0.007091
101 7 GAGTAGTGAC GGGGGGGGGG 2211757 0.008784 0.000555 0.055548 0.007040
102 8 AGCCTCTGCT GGGGGGGGGG 8025833 0.030822 0.002026 0.202591 0.025545
103 8 CCAACTGTAT GGGGGGGGGG 5249631 0.020160 0.001325 0.132513 0.016709
104 8 GCATTCCTTC GGGGGGGGGG 5018571 0.019273 0.001267 0.126680 0.015973
105 8 AGACGACAGA GGGGGGGGGG 4320655 0.016593 0.001091 0.109063 0.013752
106 8 GTGACGCACA GGGGGGGGGG 3885802 0.014923 0.000981 0.098087 0.012368
107 8 ACCAGGAATT GGGGGGGGGG 3245960 0.012466 0.000819 0.081936 0.010331
108 8 AAGGTGTACT GGGGGGGGGG 3133563 0.012034 0.000791 0.079098 0.009974
109 8 ACTCGTCCAG GGGGGGGGGG 2970142 0.011406 0.000750 0.074973 0.009453
110 8 TGTGATCGCG GGGGGGGGGG 2667138 0.010243 0.000673 0.067325 0.008489
111 8 CGGATCATTA GGGGGGGGGG 2596644 0.009972 0.000655 0.065545 0.008265
112 8 AACGCTCTGA GGGGGGGGGG 2331506 0.008954 0.000589 0.058853 0.007421
113 8 GAGTAGTGAC GGGGGGGGGG 2319363 0.008907 0.000585 0.058546 0.007382
114 8 GATAGGTGAA GGGGGGGGGG 2313511 0.008885 0.000584 0.058398 0.007363
115 8 TCACCGTGGT GGGGGGGGGG 2046059 0.007858 0.000516 0.051647 0.006512
116 8 GCAGAAGTCT GGGGGGGGGG 1982189 0.007612 0.000500 0.050035 0.006309
5. BARCODES WITH HIGH MISMATCH RATES
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No barcodes found with high mismatch rates
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FINAL STATUS CHECK
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Overall % Undetermined: 6.67%
Status: WARNING
Reason(s):
Overall undetermined 6.67% >= 5.0%